제품 > 항체 > 단일클론항체(mAb)

ABflo® 647 Rabbit anti-Mouse CD112/Nectin-2 mAb (A24091)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Flow cytometry: 1X10^6 Raw264.7 cells (Low Expression, left) and C2C12 cells (right) were surface-stained with ABflo® 647 Rabbit anti-Mouse CD112/Nectin-2 mAb (A24091, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 C2C12 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Mouse CD112/Nectin-2 mAb (A24091, 5 μl/Test, right).

Overview

Product name	ABflo® 647 Rabbit anti-Mouse CD112/Nectin-2 mAb
Catalog No.	A24091
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC64537-ABflo647

Background

Enables cell adhesion molecule binding activity and protein homodimerization activity. Involved in homophilic cell adhesion via plasma membrane adhesion molecules. Acts upstream of or within several processes, including establishment of mitochondrion localization; sperm mitochondrion organization; and spermatid development. Located in cell-cell contact zone; plasma membrane; and zonula adherens. Is expressed in brain; cochlea; enamel organ; male reproductive system; and primitive collecting duct. Human ortholog(s) of this gene implicated in Alzheimer's disease and multiple sclerosis. Orthologous to human NECTIN2 (nectin cell adhesion molecule 2).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 32-345 of mouse CD112/Nectin-2 (NP_033016.3).
Sequence	QDVRVRVLPEVRGRLGGTVELPCHLLPPTTERVSQVTWQRLDGTVVAAFHPSFGVDFPNSQFSKDRLSFVRARPETNADLRDATLAFRGLRVEDEGNYTCEFATFPNGTRRGVTWLRVIAQPENHAEAQEVTIGPQSVAVARCVSTGGRPPARITWISSLGGEAKDTQEPGIQAGTVTIISRYSLVPVGRADGVKVTCRVEHESFEEPILLPVTLSVRYPPEVSISGYDDNWYLGRSEAILTCDVRSNPEPTDYDWSTTSGVFPASAVAQGSQLLVHSVDRMVNTTFICTATNAVGTGRAEQVILVRESPSTAG
Gene ID
Swiss Prot
Synonyms	MPH; Pvr; Pvs; Cd112; Pvrl2; nectin-2
Calculated MW	57kDa
Observed MW

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane