제품 > 항체 > 다중클론항체(pAb)

CD146 Rabbit pAb (A25042)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using CD146 Rabbit pAb (A25042) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Negative control (NC): COLO 205.
Exposuretime: 90s.

Overview

Product name	CD146 Rabbit pAb
Catalog No.	A25042
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Involved in glomerular filtration and vascular wound healing. Acts upstream of or within angiogenesis. Located in external side of plasma membrane. Biomarker of chronic obstructive pulmonary disease and uveal melanoma.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 546-645 of human CD146 (NP_006491.2).
Sequence	STERKLPEPESRGVVIVAVIVCILVLAVLGAVLYFLYKKGKLPCRRSGKQEITLPPSRKSELVVEVKSDKLPEEMGLLQGSSGDKRAPGDQGEKYIDLRH
Gene ID
Swiss Prot
Synonyms	CD146; MUC18; HEMCAM; METCAM; MelCAM
Calculated MW	72kDa
Observed MW	120kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HUVEC, SK-MEL-28, Rat lung
Cellular location	Membrane, Single-pass type I membrane protein