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CD152/CTLA-4 Rabbit mAb (A22865)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - CD152/CTLA-4 Rabbit mAb (A22865)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD152/CTLA-4 using CD152/CTLA-4 Rabbit mAb (A22865) at 1:8000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - CD152/CTLA-4 Rabbit mAb (A22865)

Western blot analysis of Recombinant Human CTLA-4/CD152 Protein (RP01044), using CD152/CTLA-4 Rabbit mAb (A22865) at 1:8000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 10ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameCD152/CTLA-4 Rabbit mAb
Catalog No.A22865
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC57390
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 37-162 of human CD152/CTLA-4 (NP_005205.2).
SequenceAMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIDPEPCPDSDF
Gene ID
Swiss Prot
SynonymsCD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3; CD152/CTLA-4
Calculated MW25kDa
Observed MW25kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:9000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293F transfected with CD152/CTLA-4, Recombinant Human CTLA-4 Protein
Cellular locationCell membrane.

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ABclonal:Western blot - CD152/CTLA-4 Rabbit mAb (A22865)}

Western blot - CD152/CTLA-4 Rabbit mAb (A22865)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD152/CTLA-4 using CD152/CTLA-4 Rabbit mAb (A22865) at 1:8000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - CD152/CTLA-4 Rabbit mAb (A22865)}

Western blot - CD152/CTLA-4 Rabbit mAb (A22865)

Western blot analysis of Recombinant Human CTLA-4/CD152 Protein (RP01044), using CD152/CTLA-4 Rabbit mAb (A22865) at 1:8000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 10ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

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