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CD172a/SIRPα Rabbit mAb (A9545)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CD172a/SIRPα Rabbit mAb (A9545)

Western blot analysis of various lysates using CD172a/SIRPα Rabbit mAb (A9545) at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - CD172a/SIRPα Rabbit mAb (A9545)

Western blot analysis of various lysates using CD172a/SIRPα Rabbit mAb (A9545) at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - CD172a/SIRPα Rabbit mAb (A9545)

Immunofluorescence analysis of U-2 OS cells using CD172a/SIRPα Rabbit mAb (A9545) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCD172a/SIRPα Rabbit mAb
Catalog No.A9545
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1626
The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 395-495 of human CD172a/SIRPα (P78324).
SequenceRIRQKKAQGSTSSTRLHEPEKNAREITQDTNDITYADLNLPKGKKPAPQAAEPNNHTEYASIQTSPQPASEDTLTYADLDMVHLNRTPKQPAPKPEPSFSE
Gene ID
Swiss Prot
SynonymsBIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1; CD172a/SIRPα
Calculated MW55kDa
Observed MW85kDa(Human)/100kDa(Rat)/120kDa(Mouse)
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse brain, Rat brain, THP-1, U-937
Cellular locationMembrane, Single-pass type I membrane protein.

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ABclonal:Western blot - CD172a/SIRPα Rabbit mAb (A9545)}

Western blot - CD172a/SIRPα Rabbit mAb (A9545)

Western blot analysis of various lysates using CD172a/SIRPα Rabbit mAb (A9545) at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - CD172a/SIRPα Rabbit mAb (A9545)}

Western blot - CD172a/SIRPα Rabbit mAb (A9545)

Western blot analysis of various lysates using CD172a/SIRPα Rabbit mAb (A9545) at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - CD172a/SIRPα Rabbit mAb (A9545)}

Immunofluorescence - CD172a/SIRPα Rabbit mAb (A9545)

Immunofluorescence analysis of U-2 OS cells using CD172a/SIRPα Rabbit mAb (A9545) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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