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CD44 Rabbit pAb (A23055)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CD44 Rabbit pAb (A23055)

Western blot analysis of various lysates, using CD44 Rabbit pAb (A23055) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - CD44 Rabbit pAb (A23055)

Immunofluorescence analysis of NIH/3T3 cells using CD44 Rabbit pAb (A23055) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCD44 Rabbit pAb
Catalog No.A23055
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables hyaluronic acid binding activity and type II transforming growth factor beta receptor binding activity. Contributes to cytokine binding activity and cytokine receptor activity. Involved in several processes, including negative regulation of T cell activation; positive regulation of protein phosphorylation; and regulation of intracellular signal transduction. Acts upstream of or within several processes, including Wnt signaling pathway; morphogenesis of a branching epithelium; and wound healing involved in inflammatory response. Located in basolateral plasma membrane; external side of plasma membrane; and microvillus. Part of macrophage migration inhibitory factor receptor complex. Is expressed in several structures, including alimentary system; branchial arch; central nervous system; genitourinary system; and limb. Human ortholog(s) of this gene implicated in breast carcinoma (multiple); carcinoma (multiple); and prostate cancer. Orthologous to human CD44 (CD44 molecule (Indian blood group)).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 23-176 of mouse CD44. (NP_033981.2).
SequenceHQQIDLNVTCRYAGVFHVEKNGRYSISRTEAADLCQAFNSTLPTMDQMKLALSKGFETCRYGFIEGNVVIPRIHPNAICAANHTGVYILVTSNTSHYDTYCFNASAPPEEDCTSVTDLPNSFDGPVTITIVNRDGTRYSKKGEYRTHQEDIDAS
Gene ID
Swiss Prot
SynonymsLy-24; Pgp-1; HERMES; CD44
Calculated MW86kDa
Observed MW80-95kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:20 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse lung, Mouse spleen, Rat lung
Cellular location
Customer validation

WB(Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - CD44 Rabbit pAb (A23055)}

    Western blot - CD44 Rabbit pAb (A23055)

    Western blot analysis of various lysates, using CD44 Rabbit pAb (A23055) at 1:400 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunofluorescence - CD44 Rabbit pAb (A23055)}

    Immunofluorescence - CD44 Rabbit pAb (A23055)

    Immunofluorescence analysis of NIH/3T3 cells using CD44 Rabbit pAb (A23055) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A23055? Please let us know so that we can cite the reference in this datasheet.

    항체 (8)

    ELISA 키트 (1)

    Secondary Antibodies (25)