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CDC42EP1 Rabbit mAb (A19731)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CDC42EP1 Rabbit mAb (A19731)

Western blot analysis of various lysates using CDC42EP1 Rabbit mAb (A19731) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - CDC42EP1 Rabbit mAb (A19731)

Immunohistochemistry analysis of paraffin-embedded rat heart using CDC42EP1 Rabbit mAb (A19731) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CDC42EP1 Rabbit mAb (A19731)

Immunohistochemistry analysis of paraffin-embedded mouse heart using CDC42EP1 Rabbit mAb (A19731) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameCDC42EP1 Rabbit mAb
Catalog No.A19731
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2298
CDC42 is a member of the Rho GTPase family that regulates multiple cellular activities, including actin polymerization. The protein encoded by this gene is a CDC42 binding protein that mediates actin cytoskeleton reorganization at the plasma membrane. This protein is secreted and is primarily found in bone marrow.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 292-391 of human CDC42EP1 (Q00587).
SequenceAGLGPVAEVKSSPVGGGPRGPAGPALGRHWGAGWDGGHHYPEMDARQERVEVLPQARASWESLDEEWRAPQAGSRTPVPSTVQANTFEFADAEEDDEVKV
Gene ID
Swiss Prot
SynonymsCEP1; BORG5; MSE55; CDC42EP1
Calculated MW40kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, HT-29, HepG2
Cellular locationCytoplasm, Cytoskeleton, Cytosol, Focal adhesion, Plasma membrane

Documents

Certificate of Compliance

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ABclonal:Western blot - CDC42EP1 Rabbit mAb (A19731)}

Western blot - CDC42EP1 Rabbit mAb (A19731)

Western blot analysis of various lysates using CDC42EP1 Rabbit mAb (A19731) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - CDC42EP1 Rabbit mAb (A19731)}

Immunohistochemistry - CDC42EP1 Rabbit mAb (A19731)

Immunohistochemistry analysis of paraffin-embedded rat heart using CDC42EP1 Rabbit mAb (A19731) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CDC42EP1 Rabbit mAb (A19731)}

Immunohistochemistry - CDC42EP1 Rabbit mAb (A19731)

Immunohistochemistry analysis of paraffin-embedded mouse heart using CDC42EP1 Rabbit mAb (A19731) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)