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COPB2 Rabbit mAb (A21294)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - COPB2 Rabbit mAb (A21294)

Western blot analysis of extracts of various cell lines, using COPB2 antibody (A21294) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameCOPB2 Rabbit mAb
Catalog No.A21294
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC52626
The Golgi coatomer complex (see MIM 601924) constitutes the coat of nonclathrin-coated vesicles and is essential for Golgi budding and vesicular trafficking. It consists of 7 protein subunits, including COPB2.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 657-906 of human COPB2 (NP_004757.1).
SequenceAYQLAVEAESEQKWKQLAELAISKCQFGLAQECLHHAQDYGGLLLLATASGNANMVNKLAEGAERDGKNNVAFMSYFLQGKVDACLELLIRTGRLPEAAFLARTYLPSQVSRVVKLWRENLSKVNQKAAESLADPTEYENLFPGLKEAFVVEEWVKETHADLWPAKQYPLVTPNEERNVMEEGKDFQPSRSTAQQELDGKPASPTPVIVASHTANKEEKSLLELEVDLDNLELEDIDTTDINLDEDILDD
Gene ID
Swiss Prot
SynonymsOPDD; MCPH19; beta'-COP
Calculated MW102kDa
Observed MW102kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:3000 - 1:20000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse testis, HeLa, MCF7, Jurkat
Cellular locationCytoplasm, Golgi apparatus membrane

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    ABclonal:Western blot - COPB2 Rabbit mAb (A21294)}

    Western blot - COPB2 Rabbit mAb (A21294)

    Western blot analysis of extracts of various cell lines, using COPB2 antibody (A21294) at 1:20000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (3)

    Secondary Antibodies (25)