제품 > 항체 > 단일클론항체(mAb)

COPB2 Rabbit mAb (A22381)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates, using COPB2 antibody (A22381) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - COPB2 Rabbit mAb (A22381)

Confocal imaging of HeLa cells using COPB2 Rabbit mAb (A22381, dilution 1:100) (Red). The cells were counterstained with CD44 Rabbit mAb (A21919, dilution 1:200) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Overview

Product name	COPB2 Rabbit mAb
Catalog No.	A22381
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC52631

Background

The Golgi coatomer complex (see MIM 601924) constitutes the coat of nonclathrin-coated vesicles and is essential for Golgi budding and vesicular trafficking. It consists of 7 protein subunits, including COPB2.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 657-906 of human COPB2 (NP_004757.1).
Sequence	AYQLAVEAESEQKWKQLAELAISKCQFGLAQECLHHAQDYGGLLLLATASGNANMVNKLAEGAERDGKNNVAFMSYFLQGKVDACLELLIRTGRLPEAAFLARTYLPSQVSRVVKLWRENLSKVNQKAAESLADPTEYENLFPGLKEAFVVEEWVKETHADLWPAKQYPLVTPNEERNVMEEGKDFQPSRSTAQQELDGKPASPTPVIVASHTANKEEKSLLELEVDLDNLELEDIDTTDINLDEDILDD
Gene ID
Swiss Prot
Synonyms	OPDD; MCPH19; beta'-COP
Calculated MW	102kDa
Observed MW	102kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:20000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	MCF7, HeLa
Cellular location	COPI-coated vesicle membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus membrane, Peripheral membrane protein, cytosol