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CPS1 Rabbit mAb (A4214)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CPS1 Rabbit mAb (A4214)

Western blot analysis of various lysates using CPS1 Rabbit mAb (A4214) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - CPS1 Rabbit mAb (A4214)

Immunohistochemistry analysis of paraffin-embedded Human liver using CPS1 Rabbit mAb (A4214) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - CPS1 Rabbit mAb (A4214)

Confocal imaging of paraffin-embedded Rat liver using CPS1 Rabbit mAb (A4214, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - CPS1 Rabbit mAb (A4214)

Confocal imaging of paraffin-embedded Human liver using CPS1 Rabbit mAb (A4214, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameCPS1 Rabbit mAb
Catalog No.A4214
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0929
The mitochondrial enzyme encoded by this gene catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells. The encoded protein may also represent a core mitochondrial nucleoid protein. Three transcript variants encoding different isoforms have been found for this gene. The shortest isoform may not be localized to the mitochondrion. Mutations in this gene have been associated with carbamoyl phosphate synthetase deficiency, susceptibility to persistent pulmonary hypertension, and susceptibility to venoocclusive disease after bone marrow transplantation.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human CPS1 (NP_001866.2).
SequenceIIGNGGAPDTTALDELGLSKYLESNGIKVSGLLVLDYSKDYNHWLATKSLGQWLQEEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQPVDFVDPNKQNL
Gene ID
Swiss Prot
SynonymsPHN; GATD6; CPSASE1; CPS1
Calculated MW165kDa
Observed MW165kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:100 - 1:1000
  • IF/ICC 1:100 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Mouse liver, Rat liver
Cellular locationMitochondrion, Nucleus, nucleolus, Cell membrane.

Documents

Certificate of Compliance

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ABclonal:Western blot - CPS1 Rabbit mAb (A4214)}

Western blot - CPS1 Rabbit mAb (A4214)

Western blot analysis of various lysates using CPS1 Rabbit mAb (A4214) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - CPS1 Rabbit mAb (A4214)}

Immunohistochemistry - CPS1 Rabbit mAb (A4214)

Immunohistochemistry analysis of paraffin-embedded Human liver using CPS1 Rabbit mAb (A4214) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - CPS1 Rabbit mAb (A4214)}

Immunofluorescence - CPS1 Rabbit mAb (A4214)

Confocal imaging of paraffin-embedded Rat liver using CPS1 Rabbit mAb (A4214, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - CPS1 Rabbit mAb (A4214)}

Immunofluorescence - CPS1 Rabbit mAb (A4214)

Confocal imaging of paraffin-embedded Human liver using CPS1 Rabbit mAb (A4214, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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