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CPS1 Rabbit pAb (A21361)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - CPS1 Rabbit pAb (A21361)

Western blot analysis of various lysates, using CPS1 Rabbit pAb (A21361) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - CPS1 Rabbit pAb (A21361)

Immunofluorescence analysis of HeLa cells using CPS1 Polyclonal Antibody (A21361) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameCPS1 Rabbit pAb
Catalog No.A21361
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The mitochondrial enzyme encoded by this gene catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells. The encoded protein may also represent a core mitochondrial nucleoid protein. Three transcript variants encoding different isoforms have been found for this gene. The shortest isoform may not be localized to the mitochondrion. Mutations in this gene have been associated with carbamoyl phosphate synthetase deficiency, susceptibility to persistent pulmonary hypertension, and susceptibility to venoocclusive disease after bone marrow transplantation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 820-930 of human CPS1 (NP_001116105.1).
SequenceRMCHPSIEGFTPRLPMNKEWPSNLDLRKELSEPSSTRIYAIAKAIDDNMSLDEIEKLTYIDKWFLYKMRDILNMEKTLKGLNSESMTEETLKRAKEIGFSDKQISKCLGLT
Gene ID
Swiss Prot
SynonymsPHN; GATD6; CPSASE1
Calculated MW165kDa
Observed MW165kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU2OS(Negative control), Rat liver
Cellular locationMitochondrion, Nucleus, nucleolus

Documents

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ABclonal:Western blot - CPS1 Rabbit pAb (A21361)}

Western blot - CPS1 Rabbit pAb (A21361)

Western blot analysis of various lysates, using CPS1 Rabbit pAb (A21361) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - CPS1 Rabbit pAb (A21361)}

Immunofluorescence - CPS1 Rabbit pAb (A21361)

Immunofluorescence analysis of HeLa cells using CPS1 Polyclonal Antibody (A21361) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

Secondary Antibodies (26)