Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of extracts of Rat brain, using CRMP1 antibody (A2705) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Immunofluorescence analysis of Neuro-2a cells using CRMP1 Rabbit pAb (A2705) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
| Product name | CRMP1 Rabbit pAb |
|---|---|
| Catalog No. | A2705 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 460-540 of human CRMP1 (NP_001304.1). |
|---|---|
| Sequence | NVNKGMGRFIPRKAFPEHLYQRVKIRNKVFGLQGVSRGMYDGPVYEVPATPKYATPAPSAKSSPSKHQPPPIRNLHQSNFS |
| Gene ID | |
| Swiss Prot | |
| Synonyms | DRP1; DRP-1; CRMP-1; DPYSL1; ULIP-3 |
| Calculated MW | 62kDa |
| Observed MW | 62kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunofluorescence |
| Positive samples | Rat brain |
| Cellular location | Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle |
| Customer validation | WB(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A2705? Please let us know so that we can cite the reference in this datasheet.
