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OGDH Rabbit pAb (A12477)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - OGDH Rabbit pAb (A12477)

Western blot analysis of various lysates using OGDH Rabbit pAb (A12477) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - OGDH Rabbit pAb (A12477)

Immunohistochemistry analysis of paraffin-embedded rat brain using OGDH Rabbit pAb (A12477) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - OGDH Rabbit pAb (A12477)

Immunohistochemistry analysis of paraffin-embedded mouse brain using OGDH Rabbit pAb (A12477) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - OGDH Rabbit pAb (A12477)

Immunofluorescence analysis of U2OS cells using OGDH Rabbit pAb (A12477). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameOGDH Rabbit pAb
Catalog No.A12477
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 148-427 of human OGDH (NP_001003941.1).
SequenceDPLGILDADLDSSVPADIISSTDKLGFYGLDESDLDKVFHLPTTTFIGGQESALPLREIIRRLEMAYCQHIGVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEKRFGLEGCEVLIPALKTIIDKSSENGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFDSKLEAADEGSGDVKYHLGMYHRRINRVTDRNITLSLVANPSHLEAADPVVMGKTKAEQFYCGDTEGKKVRPRERRARQIVKAPCSSMEFRSPT
Gene ID
Swiss Prot
SynonymsE1k; E1o; KGD1; OGDC; AKGDH; OGDH2; OGDHD; OGDH-E1; OGDH
Calculated MW116kDa
Observed MW116kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse kidney, Mouse heart, Mouse brain
Cellular locationMitochondrion matrix

Documents

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ABclonal:Western blot - OGDH Rabbit pAb (A12477)}

Western blot - OGDH Rabbit pAb (A12477)

Western blot analysis of various lysates using OGDH Rabbit pAb (A12477) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - OGDH Rabbit pAb (A12477)}

Immunohistochemistry - OGDH Rabbit pAb (A12477)

Immunohistochemistry analysis of paraffin-embedded rat brain using OGDH Rabbit pAb (A12477) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - OGDH Rabbit pAb (A12477)}

Immunohistochemistry - OGDH Rabbit pAb (A12477)

Immunohistochemistry analysis of paraffin-embedded mouse brain using OGDH Rabbit pAb (A12477) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - OGDH Rabbit pAb (A12477)}

Immunofluorescence - OGDH Rabbit pAb (A12477)

Immunofluorescence analysis of U2OS cells using OGDH Rabbit pAb (A12477). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)