제품 > 항체 > 다중클론항체(pAb)

mTOR Rabbit pAb (A11355)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using mTOR Rabbit pAb (A11355) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - mTOR Rabbit pAb (A11355)

Immunofluorescence analysis of NIH-3T3 cells using mTOR Rabbit pAb (A11355) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using mTOR Rabbit pAb (A11355) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - mTOR Rabbit pAb (A11355)

Immunoprecipitation analysis of 300ug extracts of K-562 cells using 3ug mTOR Rabbit pAb (A11355 1:50). Western blot was performed from the immunoprecipitate using mTOR Rabbit pAb (A2445) at a dilition of 1:500.

Review

Overview

Product name	mTOR Rabbit pAb
Catalog No.	A11355
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 2400-2500 of human mTOR (NP_004949.1).
Sequence	CHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQI
Gene ID
Swiss Prot
Synonyms	SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; mTOR
Calculated MW	289kDa
Observed MW	289kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	HeLa, HepG2, K-562
Cellular location	Cytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Golgi apparatus membrane, Lysosome, Mitochondrion outer membrane, Nucleus, PML body, Peripheral membrane protein
Customer validation	(HeLa cells, Human chronic myeloid leukemia cells) WB(Homo sapiens, Mus musculus, Rattus norvegicus) IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

mTOR Rabbit pAb images

Western blot - mTOR Rabbit pAb (A11355)

Immunofluorescence - mTOR Rabbit pAb (A11355)

Immunoprecipitation - mTOR Rabbit pAb (A11355)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A11355

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Reviews List

Product Protocols

References for mTOR Rabbit pAb (A11355)

Product:

mTOR Rabbit pAb

Journal:

Exp Ther Med

Application:

IF:

2.44

Species:

Homo sapiens

PMID:

33850531

Title:

Long non-coding RNA ADAMTS9-AS2 inhibits liver cancer cell proliferation, migration and invasion

References for mTOR Rabbit pAb (A11355)

Product:

mTOR Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.89

Species:

Mus musculus

PMID:

36521060

Title:

Gut-Derived Metabolites from Dietary Tryptophan Supplementation Quench Intestinal Inflammation through the AMPK-SIRT1-Autophagy Pathway

References for mTOR Rabbit pAb (A11355)

Product:

mTOR Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

IF:

5.19

Species:

Mus musculus

PMID:

36842723

Title:

Hydroxysafflor yellow a confers neuroprotection against acute traumatic brain injury by modulating neuronal autophagy to inhibit NLRP3 inflammasomes

References for mTOR Rabbit pAb (A11355)

Product:

mTOR Rabbit pAb

Journal:

Mediators of inflammation

Application:

IF:

4.52

Species:

Homo sapiens

PMID:

36816744

Title:

Inhibitory Effect of Jinwujiangu Prescription on Peripheral Blood Osteoclasts in Patients with Rheumatoid Arthritis and the Relevant Molecular Mechanism - PMC

References for mTOR Rabbit pAb (A11355)

Product:

mTOR Rabbit pAb

Journal:

Cellular oncology (Dordrecht)

Application:

IF:

7.05

Species:

Homo sapiens

PMID:

37782406

Title:

FGF19 promotes nasopharyngeal carcinoma progression by inducing angiogenesis via inhibiting TRIM21-mediated ANXA2 ubiquitination

References for mTOR Rabbit pAb (A11355)

Product:

mTOR Rabbit pAb

Journal:

Advanced science

Application:

IF:

15.1

Species:

Homo sapiens

PMID:

38041494

Title:

mTORC1 Mediates Biphasic MechanoResponse to Orchestrate AdhesionDependent Cell Growth and Anoikis Resistance

References for mTOR Rabbit pAb (A11355)

Product:

mTOR Rabbit pAb

Journal:

Cancers

Application:

IF:

6.57

Species:

Homo sapiens

PMID:

36428630

Title:

UCHL5 Promotes Proliferation and Migration of Bladder Cancer Cells by Activating c-Myc via AKT/mTOR Signaling - PMC

References for mTOR Rabbit pAb (A11355)

Product:

mTOR Rabbit pAb

Journal:

Cells

Application:

IF:

7.66

Species:

Homo sapiens

PMID:

36429034

Title:

LTB4R Promotes the Occurrence and Progression of Clear Cell Renal Cell Carcinoma (ccRCC) by Regulating the AKT/mTOR Signaling Pathway - PMC

References for mTOR Rabbit pAb (A11355)

Product:

mTOR Rabbit pAb

Journal:

Gut microbes

Application:

IF:

12.2

Species:

Homo sapiens

PMID:

38630006

Title:

Sialic acid-based probiotic intervention in lactating mothers improves the neonatal gut microbiota and immune responses by regulating sialylated milk oligosaccharide synthesis via the gut-breast axis - PMC

References for mTOR Rabbit pAb (A11355)

Product:

mTOR Rabbit pAb

Journal:

Journal of leukocyte biology

Application:

IF:

5.5

Species:

Mus musculus

PMID:

38513294

Title:

Macropinocytic cups function as signal platforms for the mTORC2-AKT pathway to modulate LPS-induced cytokine expression in macrophages

References for mTOR Rabbit pAb (A11355)

Product:

mTOR Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.6

Species:

Rattus norvegicus

PMID:

38694911

Title:

Bioactive components and potential mechanisms of Biqi Capsule in the treatment of osteoarthritis: based on chondroprotective and anti-inflammatory activity - PMC

References for mTOR Rabbit pAb (A11355)

Product:

mTOR Rabbit pAb

Journal:

Microbial pathogenesis

Application:

IF:

3.8

Species:

Homo sapiens

PMID:

38705218