제품 > 항체 > 다중클론항체(pAb)

GBP2 Rabbit pAb (A12994)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using GBP2 Rabbit pAb (A12994) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

Western blot analysis of lysates from 293T cells, using GBP2 Rabbit pAb (A12994) at 1:1000 dilution.293T cells treated with different concentrations of IFNA1.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - GBP2 Rabbit pAb (A12994)

Immunofluorescence analysis of L929 cells using GBP2 Rabbit pAb (A12994) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	GBP2 Rabbit pAb
Catalog No.	A12994
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene belongs to the guanine-binding protein (GBP) family, which includes interferon-induced proteins that can bind to guanine nucleotides (GMP, GDP and GTP). The encoded protein is a GTPase which hydrolyzes GTP, predominantly to GDP. The protein may play a role as a marker of squamous cell carcinomas.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 410-520 of human GBP2 (NP_004111.2).
Sequence	QDIFGPLEEDVKQGTFSKPGGYRLFTQKLQELKNKYYQVPRKGIQAKEVLKKYLESKEDVADALLQTDQSLSEKEKAIEVERIKAESAEAAKKMLEEIQKKNEEMMEQKEK
Gene ID
Swiss Prot
Synonyms	GBP2
Calculated MW	67kDa
Observed MW	67kDa/70kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	293T, A375, 293T treated with IFNA1
Cellular location	Cytoplasm, Golgi apparatus membrane, Lipid-anchor, Membrane, perinuclear region
Customer validation	WB(Mus musculus, Homo sapiens) IF(Mus musculus)