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Bcl-XL Rabbit pAb (A0209)

Publications (22) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Bcl-XL Rabbit pAb (A0209)

Western blot analysis of various lysates using Bcl-XL Rabbit pAb (A0209) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Bcl-XL Rabbit pAb (A0209)

Immunohistochemistry analysis of paraffin-embedded Human liver using Bcl-XL Rabbit pAb (A0209) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - Bcl-XL Rabbit pAb (A0209)

Immunofluorescence analysis of C6 cells using Bcl-XL Rabbit pAb (A0209) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameBcl-XL Rabbit pAb
Catalog No.A0209
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human Bcl-XL (NP_001304848.1).
SequenceMSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREV
Gene ID
Swiss Prot
SynonymsBCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S; Bcl-XL
Calculated MW26kDa
Observed MW30kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesOVCAR3, Rat lung, Rat spleen
Cellular locationCytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Mitochondrion inner membrane, Mitochondrion matrix, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein, centrosome, cytoskeleton, cytosol, microtubule organizing center, secretory vesicle, synaptic vesicle membrane.
Customer validation

WB(Homo sapiens, Gallus gallus, Rattus norvegicus, Mus musculus, Other)

IHC(Rattus norvegicus)

IHC(Homo sapiens)

IF(Gallus gallus)

IF(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Bcl-XL Rabbit pAb (A0209)}

Western blot - Bcl-XL Rabbit pAb (A0209)

Western blot analysis of various lysates using Bcl-XL Rabbit pAb (A0209) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Bcl-XL Rabbit pAb (A0209)}

Immunohistochemistry - Bcl-XL Rabbit pAb (A0209)

Immunohistochemistry analysis of paraffin-embedded Human liver using Bcl-XL Rabbit pAb (A0209) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - Bcl-XL Rabbit pAb (A0209)}

Immunofluorescence - Bcl-XL Rabbit pAb (A0209)

Immunofluorescence analysis of C6 cells using Bcl-XL Rabbit pAb (A0209) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

Secondary Antibodies (26)