제품 > 항체 > 다중클론항체(pAb)

Hsp90α Rabbit pAb (A0365)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Hsp90α Rabbit pAb (A0365) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - Hsp90α Rabbit pAb (A0365)

Immunofluorescence analysis of U2OS cells using Hsp90α Rabbit pAb (A0365).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

Review

Overview

Product name	Hsp90α Rabbit pAb
Catalog No.	A0365
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 433-732 of human Hsp90α (NP_005339.3).
Sequence	NYKKFYEQFSKNIKLGIHEDSQNRKKLSELLRYYTSASGDEMVSLKDYCTRMKENQKHIYYITGETKDQVANSAFVERLRKHGLEVIYMIEPIDEYCVQQLKEFEGKTLVSVTKEGLELPEDEEEKKKQEEKKTKFENLCKIMKDILEKKVEKVVVSNRLVTSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMAAKKHLEINPDHSIIETLRQKAEADKNDKSVKDLVILLYETALLSSGFSLEDPQTHANRIYRMIKLGLGIDEDDPTADDTSAAVTEEMPPLEGDDDTSRMEEVD
Gene ID
Swiss Prot
Synonyms	EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p; Hsp90α
Calculated MW	85kDa
Observed MW	90kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:100 - 1:500 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	U-251MG, HeLa, NIH/3T3, Rat brain, PC-12
Cellular location	Cell membrane, Cytoplasm, Melanosome.
Customer validation	(Mouse Embryonic Stem Cells, Human liver cell, 293T) WB(Homo sapiens, Mus musculus) IF(Mus musculus) IF(Mus musculus)

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Research Area

Hsp90α Rabbit pAb images

Western blot - Hsp90α Rabbit pAb (A0365)

Immunofluorescence - Hsp90α Rabbit pAb (A0365)

Immunofluorescence analysis of U2OS cells using Hsp90α Rabbit pAb (A0365).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution.

* For research use only. Not for therapeutic or diagnostic purposes.

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Product Protocols

References for Hsp90α Rabbit pAb (A0365)

Product:

Hsp90α Rabbit pAb

Journal:

Journal of biological chemistry

Application:

IF:

5.157

Species:

Homo sapiens

PMID:

27489107

Title:

Regulation of Ubiquitin-like with Plant Homeodomain and RING Finger Domain 1 (UHRF1) Protein Stability by Heat Shock Protein 90 Chaperone Machinery

References for Hsp90α Rabbit pAb (A0365)

Product:

Hsp90α Rabbit pAb

Journal:

Journal of Animal Science

Application:

IF:

3.159

Species:

Mus musculus

PMID:

29800308

Title:

Heat shock protein 90α couples with the MAPK signaling pathway to determine meiotic maturation of porcine oocytes

References for Hsp90α Rabbit pAb (A0365)

Product:

Hsp90α Rabbit pAb

Journal:

Journal of virology

Application:

IF:

5.103

Species:

Homo sapiens

PMID:

30541828

Title:

Autophagy benefits the replication of influenza A virus in vitro.

References for Hsp90α Rabbit pAb (A0365)

Product:

Hsp90α Rabbit pAb

Journal:

Biomaterials

Application:

IF:

12.479

Species:

Homo sapiens

PMID:

26646625

Title:

Gold-nanorods-siRNA nanoplex for improved photothermal therapy by gene silencing

References for Hsp90α Rabbit pAb (A0365)

Product:

Hsp90α Rabbit pAb

Journal:

Environ Pollut

Application:

IF:

8.071

Species:

Mus musculus

PMID:

34699919

Title:

Lycopene mitigates DEHP-induced hepatic mitochondrial quality control disorder via regulating SIRT1/PINK1/mitophagy axis and mitochondrial unfolded protein response

References for Hsp90α Rabbit pAb (A0365)

Product:

Hsp90α Rabbit pAb

Journal:

Autophagy

Application:

IF:

13.39

Species:

Homo sapiens

PMID:

36779599