제품 > 항체 > 단일클론항체(mAb)

Cadherin-13 (CDH13) Rabbit mAb (A3244)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of extracts of Mouse heart, using Cadherin-13 (CDH13) Rabbit mAb (A3244) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - Cadherin-13 (CDH13) Rabbit mAb (A3244)

Immunohistochemistry analysis of Cadherin-13 (CDH13) in paraffin-embedded human cervix cancer tissue using Cadherin-13 (CDH13) Rabbit mAb (A3244) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Cadherin-13 (CDH13) in paraffin-embedded human colon carcinoma tissue using Cadherin-13 (CDH13) Rabbit mAb (A3244) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Cadherin-13 (CDH13) in paraffin-embedded mouse kidney tissue using Cadherin-13 (CDH13) Rabbit mAb (A3244) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product name	Cadherin-13 (CDH13) Rabbit mAb
Catalog No.	A3244
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1931

Background

This gene encodes a member of the cadherin superfamily. The encoded protein is localized to the surface of the cell membrane and is anchored by a GPI moiety, rather than by a transmembrane domain. The protein lacks the cytoplasmic domain characteristic of other cadherins, and so is not thought to be a cell-cell adhesion glycoprotein. This protein acts as a negative regulator of axon growth during neural differentiation. It also protects vascular endothelial cells from apoptosis due to oxidative stress, and is associated with resistance to atherosclerosis. The gene is hypermethylated in many types of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 101-200 of human Cadherin-13 (CDH13) (P55290).
Sequence	PHAEDMAELVIVGGKDIQGSLQDIFKFARTSPVPRQKRSIVVSPILIPENQRQPFPRDVGKVVDSDRPERSKFRLTGKGVDQEPKGIFRINENTGSVSVT
Gene ID
Swiss Prot
Synonyms	CDHH; P105
Calculated MW	78kDa
Observed MW	105kDa/130kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	Mouse heart
Cellular location	Cell membrane, GPI-anchor, Lipid-anchor
Customer validation	IHC(Homo sapiens)