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Calpain 2 Rabbit mAb (A4066)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Calpain 2 Rabbit mAb (A4066)

Western blot analysis of various lysates using Calpain 2 Rabbit mAb (A4066) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product nameCalpain 2 Rabbit mAb
Catalog No.A4066
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0891
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 600-700 of human Calpain 2 (P17655).
SequenceLWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKMPCQLHQVIVARFADDQLIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIELDLISWLCFSVL
Gene ID
Swiss Prot
SynonymsCANP2; mCANP; CANPL2; CANPml; Calpain 2
Calculated MW80kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, Mouse lung, Mouse brain, Mouse kidney, Rat lung, Rat brain, Rat kidney
Cellular locationCell membrane, Cytoplasm.
Customer validation

WB(Rattus norvegicus)

IF(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - Calpain 2 Rabbit mAb (A4066)}

Western blot - Calpain 2 Rabbit mAb (A4066)

Western blot analysis of various lysates using Calpain 2 Rabbit mAb (A4066) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)