제품 > 항체 > 다중클론항체(pAb)

Calpain 2 Rabbit pAb (A2671)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Calpain 2 Rabbit pAb (A2671)

Western blot analysis of various lysates using Calpain 2 Rabbit pAb (A2671).
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Western blot - Calpain 2 Rabbit pAb (A2671)

Western blot analysis of various lysates using Calpain 2 Rabbit pAb (A2671) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - Calpain 2 Rabbit pAb (A2671)

Immunohistochemistry analysis of paraffin-embedded Human liver using Calpain 2 Rabbit pAb (A2671) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Calpain 2 Rabbit pAb (A2671)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Calpain 2 Rabbit pAb (A2671) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Calpain 2 Rabbit pAb (A2671)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using Calpain 2 Rabbit pAb (A2671) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameCalpain 2 Rabbit pAb
Catalog No.A2671
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 520-700 of human Calpain 2 (NP_001739.2).
SequenceLEEFDISEDDIDDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKMPCQLHQVIVARFADDQLIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIELDLISWLCFSVL
Gene ID
Swiss Prot
SynonymsCANP2; mCANP; CANPL2; CANPml; Calpain 2
Calculated MW80kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, Mouse brain, Mouse skin
Cellular locationCell membrane, Cytoplasm.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Calpain 2 Rabbit pAb (A2671)}

Western blot - Calpain 2 Rabbit pAb (A2671)

Western blot analysis of various lysates using Calpain 2 Rabbit pAb (A2671).
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - Calpain 2 Rabbit pAb (A2671)}

Western blot - Calpain 2 Rabbit pAb (A2671)

Western blot analysis of various lysates using Calpain 2 Rabbit pAb (A2671) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - Calpain 2 Rabbit pAb (A2671)}

Immunohistochemistry - Calpain 2 Rabbit pAb (A2671)

Immunohistochemistry analysis of paraffin-embedded Human liver using Calpain 2 Rabbit pAb (A2671) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Calpain 2 Rabbit pAb (A2671)}

Immunohistochemistry - Calpain 2 Rabbit pAb (A2671)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Calpain 2 Rabbit pAb (A2671) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Calpain 2 Rabbit pAb (A2671)}

Immunohistochemistry - Calpain 2 Rabbit pAb (A2671)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using Calpain 2 Rabbit pAb (A2671) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)