제품 > 항체 > 단일클론항체(mAb)

Cleaved Gasdermin B（C Terminal） Rabbit mAb (A24342)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from GSDMB-C-6-his protein, using Cleaved Gasdermin B（C Terminal） Rabbit mAb (A24342) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.3s.
WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Western blot analysis of lysates from 293T GSDMB stable cells and 293T GSDMB stable cells transfected with GZMA using Cleaved Gasdermin B（C Terminal） Rabbit mAb (A24342) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Review

Overview

Product name	Cleaved Gasdermin B（C Terminal） Rabbit mAb
Catalog No.	A24342
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC63400

Background

This gene encodes a member of the gasdermin-domain containing protein family. Other gasdermin-family genes are implicated in the regulation of apoptosis in epithelial cells, and are linked to cancer. Alternative splicing and the use of alternative promoters results in multiple transcript variants. Additional variants have been described, but they are candidates for nonsense-mediated mRNA decay (NMD) and are unlikely to be protein-coding.

Immunogen information

Immunogen	A synthetic peptide corresponding to residues surrounding Ser245 of human Gasdermin B (NP_001159430.1).
Sequence	GKSLG
Gene ID
Swiss Prot
Synonyms	GSDML; PP4052; GSDMB-1; PRO2521; Cleaved Gasdermin B（C Terminal）
Calculated MW	18kDa/45kDa/46kDa/47kDa
Observed MW	16kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	GSDMB-C-6-his, 293T GSDMB stable cells transfected with GZMA
Cellular location	Cytoplasm
Customer validation	(HEK293T cells)