제품 > 항체 > 다중클론항체(pAb)

Cold1 Rabbit pAb (A20713)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - Cold1 Rabbit pAb (A20713)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using Cold1 Rabbit pAb (A20713) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

Overview

Product nameCold1 Rabbit pAb
Catalog No.A20713
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Involved in chilling tolerance. Interacts with the G-protein alpha subunit GPA1 to activate the calcium channel for sensing low temperature and to accelerate G-protein GTPase activity.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 90-260 of Oryza sativa Cold1. (Q7X7S8).
SequenceVFVLPYYHCYLLLRNSGVRRERALLVAALFLLVFLYGFWRMGIHFPMPSPEKGFFTMPQLVSRIGVIGVSVMAVLSGFGAVNLPYSYLSLFIREIDEKDIKTLERQLMQSMETCIAKKKKIVLSKMEMERIQGSEEKLKARSFLKRIVGTVVRSVQEDQTEQDIKSLDAEV
Gene ID
Swiss Prot
SynonymsCOLD1; OsJ_16035; Cold1
Calculated MW53kDa
Observed MW53kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesleaves, stems, spikes
Cellular locationplasma membrane
    ABclonal:Western blot - Cold1 Rabbit pAb (A20713)}

    Western blot - Cold1 Rabbit pAb (A20713)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using Cold1 Rabbit pAb (A20713) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)