제품 > 항체 > 다중클론항체(pAb)

Collagen X/COL10A1 Rabbit pAb (A18604)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Western blot analysis of lysates from Rat lung, using Collagen X/COL10A1 Rabbit pAb (A18604) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Collagen X/COL10A1 Rabbit pAb (A18604)

Immunohistochemistry analysis of paraffin-embedded Human colon using Collagen X/COL10A1 Rabbit pAb (A18604) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product name	Collagen X/COL10A1 Rabbit pAb
Catalog No.	A18604
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 521-680 of human Collagen X/COL10A1 (NP_000484.2).
Sequence	MPEGFIKAGQRPSLSGTPLVSANQGVTGMPVSAFTVILSKAYPAIGTPIPFDKILYNRQQHYDPRTGIFTCQIPGIYYFSYHVHVKGTHVWVGLYKNGTPVMYTYDEYTKGYLDQASGSAIIDLTENDQVWLQLPNAESNGLYSSEYVHSSFSGFLVAPM
Gene ID
Swiss Prot
Synonyms	COL10A1; collagen alpha-1(X) chain; Collagen X/COL10A1
Calculated MW	66kDa
Observed MW	66kDa

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	Rat lung
Cellular location	Secreted, extracellular matrix, extracellular space.
Customer validation	WB(Gallus gallus, Mus musculus, Homo sapiens) IHC(Rattus norvegicus, Homo sapiens) IHC(Mus musculus, Gallus gallus) IF(Gallus gallus) IF(Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

연구세포 생물학 & 발달 생물학세포외 기질콜라겐

Collagen X/COL10A1 Rabbit pAb images

Western blot - Collagen X/COL10A1 Rabbit pAb (A18604)

Immunohistochemistry - Collagen X/COL10A1 Rabbit pAb (A18604)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Collagen X/COL10A1 Rabbit pAb (A18604)

Product:

Collagen X/COL10A1 Rabbit pAb

Journal:

Poultry science

Application:

IF:

4.4

Species:

Gallus gallus

PMID:

36370660

Title:

Primary growth plate chondrocyte isolation, culture, and characterization from the modern broiler - PMC

References for Collagen X/COL10A1 Rabbit pAb (A18604)

Product:

Collagen X/COL10A1 Rabbit pAb

Journal:

ACS applied materials & interfaces

Application:

IHC

IF:

8.3

Species:

Rattus norvegicus

PMID:

36779653

Title:

3D Bioprinting Using Synovium-Derived MSC-Laden Photo-Cross-Linked ECM Bioink for Cartilage Regeneration

References for Collagen X/COL10A1 Rabbit pAb (A18604)

Product:

Collagen X/COL10A1 Rabbit pAb

Journal:

Advanced healthcare materials

Application:

IHC

IF:

Species:

Homo sapiens

PMID:

37462089

Title:

A BioglassPoly (lacticcoglycolic Acid) Scaffold@ Fibrin Hydrogel Construct to Support Endochondral Bone Formation

References for Collagen X/COL10A1 Rabbit pAb (A18604)

Product:

Collagen X/COL10A1 Rabbit pAb

Journal:

Bone

Application:

WB, IHC

IF:

3.5

Species:

Mus musculus

PMID:

34530171

Title:

Mettl3 inhibits the apoptosis and autophagy of chondrocytes in inflammation through mediating Bcl2 stability via Ythdf1-mediated m6A modification

References for Collagen X/COL10A1 Rabbit pAb (A18604)

Product:

Collagen X/COL10A1 Rabbit pAb

Journal:

Journal of bone and mineral metabolism

Application:

WB, IHC

IF:

2.4

Species:

Mus musculus

PMID:

28357594

Title:

Knockdown of Ggps1 in chondrocyte expedites fracture healing by accelerating the progression of endochondral ossification in mice

References for Collagen X/COL10A1 Rabbit pAb (A18604)

Product:

Collagen X/COL10A1 Rabbit pAb

Journal:

Bone

Application:

WB, IHC

IF:

3.5

Species:

Mus musculus

PMID:

35981698

Title:

Mettl3 regulates hypertrophic differentiation of chondrocytes through modulating Dmp1 mRNA via Ythdf1-mediated m6A modification

References for Collagen X/COL10A1 Rabbit pAb (A18604)

Product:

Collagen X/COL10A1 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

WB, IHC, IF

IF:

5.6

Species:

Gallus gallus

PMID:

37446153

Title:

The Effect of miR-140-5p with HDAC4 towards Growth and Differentiation Signaling of Chondrocytes in Thiram-Induced Tibial Dyschondroplasia - PMC

References for Collagen X/COL10A1 Rabbit pAb (A18604)

Product:

Collagen X/COL10A1 Rabbit pAb

Journal:

Frontiers in bioengineering and biotechnology

Application:

IF:

4.3

Species:

Rattus norvegicus

PMID:

38600948

Title:

Periodic static compression of micro-strain pattern regulates endochondral bone formation - PMC

References for Collagen X/COL10A1 Rabbit pAb (A18604)

Product:

Collagen X/COL10A1 Rabbit pAb

Journal:

Scientific reports

Application:

IF:

3.8

Species:

Homo sapiens

PMID:

38702382

Title:

Extremely low frequency-electromagnetic fields promote chondrogenic differentiation of adipose-derived mesenchymal stem cells through a conventional genetic program - PMC