제품 > 항체 > 다중클론항체(pAb)

Complement C3 Rabbit pAb (A13283)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Immunofluorescence analysis of paraffin-embedded rat liver using Complement C3 Rabbit pAb (A13283) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of paraffin-embedded human liver cancer using Complement C3 Rabbit pAb (A13283) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of paraffin-embedded mouse liver using Complement C3 Rabbit pAb (A13283) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Complement C3 Rabbit pAb
Catalog No.	A13283
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1364-1663 of human Complement C3 (NP_000055.2).
Sequence	KVTIKPAPETEKRPQDAKNTMILEICTRYRGDQDATMSILDISMMTGFAPDTDDLKQLANGVDRYISKYELDKAFSDRNTLIIYLDKVSHSEDDCLAFKVHQYFNVELIQPGAVKVYAYYNLEESCTRFYHPEKEDGKLNKLCRDELCRCAEENCFIQKSDDKVTLEERLDKACEPGVDYVYKTRLVKVQLSNDFDEYIMAIEQTIKSGSDEVQVGQQRTFISPIKCREALKLEEKKHYLMWGLSSDFWGEKPNLSYIIGKDTWVEHWPEEDECQDEENQKQCQDLGAFTESMVVFGCPN
Gene ID
Swiss Prot
Synonyms	ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p; Complement C3
Calculated MW	187kDa
Observed MW

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Immunofluorescence
Positive samples	HuH-7
Cellular location	Secreted.
Customer validation	IHC(Rattus norvegicus, Canis lupus familiaris, Mus musculus) IF(Canis lupus familiaris, Mus musculus, Rattus norvegicus) WB(Homo sapiens, Mus musculus, Rattus norvegicus) IF(Rattus norvegicus, Mus musculus) IHC(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

연구신호 변환G 단백질 신호전달G-단백질 결합 수용체(GPCR)

Complement C3 Rabbit pAb images

Immunofluorescence - Complement C3 Rabbit pAb (A13283)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Complement C3 Rabbit pAb (A13283)

Product:

Complement C3 Rabbit pAb

Journal:

Clinical and experimental immunology

Application:

IHC

IF:

4.33

Species:

Rattus norvegicus

PMID:

31344254

Title:

Transcriptomes in rat sciatic nerves at different stages of experimental autoimmune neuritis determined by RNA sequencing.

References for Complement C3 Rabbit pAb (A13283)

Product:

Complement C3 Rabbit pAb

Journal:

Int J Med Sci

Application:

IHC, IF

IF:

3.73

Species:

Canis lupus familiaris

PMID:

34975308

Title:

Early Toll-like receptor 4 inhibition improves immune dysfunction in the hippocampus after hypoxic-ischemic brain damage

References for Complement C3 Rabbit pAb (A13283)

Product:

Complement C3 Rabbit pAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

7.31

Species:

Homo sapiens

PMID:

36046683

Title:

Autophagy-Mediated Inflammatory Cytokine Secretion in Sporadic ALS Patient iPSC-Derived Astrocytes - PMC

References for Complement C3 Rabbit pAb (A13283)

Product:

Complement C3 Rabbit pAb

Journal:

Frontiers in aging neuroscience

Application:

IF:

5.7

Species:

Rattus norvegicus

PMID:

35663575

Title:

Mesenchymal Stem Cells Improve Cognitive Impairment and Reduce Aβ Deposition via Promoting AQP4 Polarity and Relieving Neuroinflammation in Rats With Chronic Hypertension-Induced Cerebral Small-Vessel Disease - PMC

References for Complement C3 Rabbit pAb (A13283)

Product:

Complement C3 Rabbit pAb

Journal:

Journal of neuroinflammation

Application:

WB, IF

IF:

9.587

Species:

Mus musculus

PMID:

32665021

Title:

Microglia induce the transformation of A1/A2 reactive astrocytes via the CXCR7/PI3K/Akt pathway in chronic post-surgical pain

References for Complement C3 Rabbit pAb (A13283)

Product:

Complement C3 Rabbit pAb

Journal:

Life Sciences

Application:

WB, IHC

IF:

5.037

Species:

Rattus norvegicus

PMID:

32360572

Title:

Hyperoside attenuates pregnancy loss through activating autophagy and suppressing inflammation in a rat model

References for Complement C3 Rabbit pAb (A13283)

Product:

Complement C3 Rabbit pAb

Journal:

PloS one

Application:

WB, IF

IF:

3.75

Species:

Rattus norvegicus

PMID:

36315519

Title:

2-Bromopalmitate decreases spinal inflammation and attenuates oxaliplatin-induced neuropathic pain via reducing Drp1-mediated mitochondrial dysfunction - PMC

References for Complement C3 Rabbit pAb (A13283)

Product:

Complement C3 Rabbit pAb

Journal:

European Journal of Pharmacology

Application:

IF:

Species:

Rattus norvegicus

PMID:

35777441

Title:

Notch signaling activation contributes to paclitaxel-induced neuropathic pain via activation of A1 astrocytes

References for Complement C3 Rabbit pAb (A13283)

Product:

Complement C3 Rabbit pAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Mus musculus

PMID:

38877492

Title:

Evaluating the pro-survival potential of apoptotic bodies derived from 2D- and 3D- cultured adipose stem cells in ischaemic flaps - PMC

References for Complement C3 Rabbit pAb (A13283)

Product:

Complement C3 Rabbit pAb

Journal:

European journal of pharmacology

Application:

IF:

Species:

Mus musculus

PMID:

38839028

Title:

The PP2A Inhibitor LB-100 Mitigates Lupus Nephritis by Suppressing Tertiary Lymphoid Structure Formation

References for Complement C3 Rabbit pAb (A13283)

Product:

Complement C3 Rabbit pAb

Journal:

Scientific reports

Application:

IHC

IF:

4.6

Species:

Mus musculus

PMID:

39019902

Title:

Delivery of CDNF by AAV-mediated gene transfer protects dopamine neurons and regulates ER stress and inflammation in an acute MPTP mouse model of Parkinsons disease - PMC