제품 > 항체 > 다중클론항체(pAb)

Cullin 4A Rabbit pAb (A2882)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Cullin 4A Rabbit pAb (A2882) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunoprecipitation - Cullin 4A Rabbit pAb (A2882)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg Cullin 4A antibody (A2882). Western blot was performed from the immunoprecipitate using Cullin 4A antibody (A2882) at a dilution of 1:1000.

Overview

Product name	Cullin 4A Rabbit pAb
Catalog No.	A2882
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins (Liu et al., 2009 [PubMed 19481525]).

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-300 of human Cullin 4A (NP_001008895.1).
Sequence	MADEAPRKGSFSALVGRTNGLTKPAALAAAPAKPGGAGGSKKLVIKNFRDRPRLPDNYTQDTWRKLHEAVRAVQSSTSIRYNLEELYQAVENLCSHKVSPMLYKQLRQACEDHVQAQILPFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTYVLQNSTLPSIWDMGLELFRTHIISDKMVQSKTIDGILLLIERERSGEAVDRSLLRSLLGMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQEREVPEYLNHVSKRLEEEGDRVITYLDHSTQKPLIACVEKQLLGEHLT
Gene ID
Swiss Prot
Synonyms	CUL4A; cullin-4A; Cullin 4A
Calculated MW	88kDa
Observed MW	80kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:100 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	K-562, BT-474, HeLa, Mouse testis
Cellular location	nucleoplasm, nucleus.
Customer validation	WB(Homo sapiens, Mus musculus) Co-IP(Homo sapiens, Mus musculus)