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Cytokeratin 16 (KRT16) Rabbit mAb (A8690)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Cytokeratin 16 (KRT16) Rabbit mAb (A8690)

Western blot analysis of lysates from Mouse large intestine, using Cytokeratin 16 (KRT16) Rabbit mAb (A8690) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Cytokeratin 16 (KRT16) Rabbit mAb (A8690)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Cytokeratin 16 (KRT16) Rabbit mAb (A8690) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Cytokeratin 16 (KRT16) Rabbit mAb (A8690)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Cytokeratin 16 (KRT16) Rabbit mAb (A8690) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - Cytokeratin 16 (KRT16) Rabbit mAb (A8690)

Confocal imaging of paraffin-embedded Human skin using Cytokeratin 16 (KRT16) Rabbit mAb (A8690, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameCytokeratin 16 (KRT16) Rabbit mAb
Catalog No.A8690
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1783
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cytokeratin 16 (KRT16) (P08779).
SequenceMTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSRFSSGGACGLGGGYGGGFSSSSSFGSGFGGGYGGGLGAGFGGGLGA
Gene ID
Swiss Prot
SynonymsK16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A; Cytokeratin 16 (KRT16)
Calculated MW51kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:500 - 1:2000
  • IF/ICC 1:100 - 1:400
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse large intestine
Cellular locationcytoskeleton, cytosol, extracellular exosome, nucleus.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - Cytokeratin 16 (KRT16) Rabbit mAb (A8690)}

Western blot - Cytokeratin 16 (KRT16) Rabbit mAb (A8690)

Western blot analysis of lysates from Mouse large intestine, using Cytokeratin 16 (KRT16) Rabbit mAb (A8690) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Cytokeratin 16 (KRT16) Rabbit mAb (A8690)}

Immunohistochemistry - Cytokeratin 16 (KRT16) Rabbit mAb (A8690)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Cytokeratin 16 (KRT16) Rabbit mAb (A8690) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Cytokeratin 16 (KRT16) Rabbit mAb (A8690)}

Immunohistochemistry - Cytokeratin 16 (KRT16) Rabbit mAb (A8690)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Cytokeratin 16 (KRT16) Rabbit mAb (A8690) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - Cytokeratin 16 (KRT16) Rabbit mAb (A8690)}

Immunofluorescence - Cytokeratin 16 (KRT16) Rabbit mAb (A8690)

Confocal imaging of paraffin-embedded Human skin using Cytokeratin 16 (KRT16) Rabbit mAb (A8690, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)