제품 > 항체 > 다중클론항체(pAb)

Cytokeratin 16 (KRT16) Rabbit pAb (A7493)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)

Western blot analysis of various lysates using Cytokeratin 16 (KRT16) Rabbit pAb (A7493) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Cytokeratin 16 (KRT16) Rabbit pAb (A7493) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)

Immunohistochemistry analysis of paraffin-embedded Human skin using Cytokeratin 16 (KRT16) Rabbit pAb (A7493) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)

Immunofluorescence analysis of A-549 cells using Cytokeratin 16 (KRT16) Rabbit pAb (A7493) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)

Immunofluorescence analysis of paraffin-embedded rat skin using Cytokeratin 16 (KRT16) Rabbit pAb (A7493) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)

Immunofluorescence analysis of paraffin-embedded mouse skin using Cytokeratin 16 (KRT16) Rabbit pAb (A7493) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCytokeratin 16 (KRT16) Rabbit pAb
Catalog No.A7493
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 190-430 of human Cytokeratin 16 (KRT16) (NP_005548.2).
SequenceLQIDNARLAADDFRTKYEHELALRQTVEADVNGLRRVLDELTLARTDLEMQIEGLKEELAYLRKNHEEEMLALRGQTGGDVNVEMDAAPGVDLSRILNEMRDQYEQMAEKNRRDAETWFLSKTEELNKEVASNSELVQSSRSEVTELRRVLQGLEIELQSQLSMKASLENSLEETKGRYCMQLSQIQGLIGSVEEQLAQLRCEMEQQSQEYQILLDVKTRLEQEIATYRRLLEGEDAHLSS
Gene ID
Swiss Prot
SynonymsK16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A; Cytokeratin 16 (KRT16)
Calculated MW51kDa
Observed MW47kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, HepG2, SW620
Cellular locationcytoskeleton, cytosol, extracellular exosome, nucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)}

Western blot - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)

Western blot analysis of various lysates using Cytokeratin 16 (KRT16) Rabbit pAb (A7493) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)}

Immunohistochemistry - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Cytokeratin 16 (KRT16) Rabbit pAb (A7493) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)}

Immunohistochemistry - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)

Immunohistochemistry analysis of paraffin-embedded Human skin using Cytokeratin 16 (KRT16) Rabbit pAb (A7493) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)}

Immunofluorescence - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)

Immunofluorescence analysis of A-549 cells using Cytokeratin 16 (KRT16) Rabbit pAb (A7493) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)}

Immunofluorescence - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)

Immunofluorescence analysis of paraffin-embedded rat skin using Cytokeratin 16 (KRT16) Rabbit pAb (A7493) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)}

Immunofluorescence - Cytokeratin 16 (KRT16) Rabbit pAb (A7493)

Immunofluorescence analysis of paraffin-embedded mouse skin using Cytokeratin 16 (KRT16) Rabbit pAb (A7493) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)