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DCN Rabbit pAb (A15048)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DCN Rabbit pAb (A15048)

Western blot analysis of extracts of various cell lines, using DCN antibody (A15048) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - DCN Rabbit pAb (A15048)

Western blot analysis of extracts of HeLa cells, using DCN antibody (A15048) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - DCN Rabbit pAb (A15048)

Immunohistochemistry analysis of paraffin-embedded human colon using DCN Rabbit pAb (A15048) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - DCN Rabbit pAb (A15048)

Immunohistochemistry analysis of paraffin-embedded human placenta using DCN Rabbit pAb (A15048) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - DCN Rabbit pAb (A15048)

Immunohistochemistry analysis of paraffin-embedded human testis using DCN Rabbit pAb (A15048) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - DCN Rabbit pAb (A15048)

Immunofluorescence analysis of NIH-3T3 cells using DCN Rabbit pAb (A15048) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameDCN Rabbit pAb
Catalog No.A15048
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the small leucine-rich proteoglycan family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. This protein plays a role in collagen fibril assembly. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. This gene and the related gene biglycan are thought to be the result of a gene duplication. Mutations in this gene are associated with congenital stromal corneal dystrophy in human patients.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 31-359 of human DCN (NP_001911.1).
SequenceDEASGIGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQCSDLGLDKVPKDLPPDTTLLDLQNNKITEIKDGDFKNLKNLHALILVNNKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITKVRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMKKLSYIRIADTNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPPGHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRSAIQLGNYK
Gene ID
Swiss Prot
SynonymsCSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
Calculated MW40kDa
Observed MW45kDa/48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Mouse heart, Rat heart
Cellular locationSecreted, extracellular matrix, extracellular space

Documents

Certificate of Compliance

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ABclonal:Western blot - DCN Rabbit pAb (A15048)}

Western blot - DCN Rabbit pAb (A15048)

Western blot analysis of extracts of various cell lines, using DCN antibody (A15048) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - DCN Rabbit pAb (A15048)}

Western blot - DCN Rabbit pAb (A15048)

Western blot analysis of extracts of HeLa cells, using DCN antibody (A15048) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - DCN Rabbit pAb (A15048)}

Immunohistochemistry - DCN Rabbit pAb (A15048)

Immunohistochemistry analysis of paraffin-embedded human colon using DCN Rabbit pAb (A15048) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - DCN Rabbit pAb (A15048)}

Immunohistochemistry - DCN Rabbit pAb (A15048)

Immunohistochemistry analysis of paraffin-embedded human placenta using DCN Rabbit pAb (A15048) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - DCN Rabbit pAb (A15048)}

Immunohistochemistry - DCN Rabbit pAb (A15048)

Immunohistochemistry analysis of paraffin-embedded human testis using DCN Rabbit pAb (A15048) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - DCN Rabbit pAb (A15048)}

Immunofluorescence - DCN Rabbit pAb (A15048)

Immunofluorescence analysis of NIH-3T3 cells using DCN Rabbit pAb (A15048) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

ELISA 키트 (1)

재조합 단백질 (2)

Secondary Antibodies (26)