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Decorin Rabbit mAb (A26852)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Decorin Rabbit mAb (A26852)

Western blot analysis of lysates from Rat uterus using Decorin Rabbit mAb (A26852) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Decorin Rabbit mAb (A26852)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Decorin Rabbit mAb (A26852) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Decorin Rabbit mAb (A26852)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Decorin Rabbit mAb (A26852) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - Decorin Rabbit mAb (A26852)

Confocal imaging of paraffin-embedded Human ovary tissue using Decorin Rabbit mAb (A26852, dilution 1:3360) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - Decorin Rabbit mAb (A26852)

Confocal imaging of paraffin-embedded Human prostate cancer tissue using Decorin Rabbit mAb (A26852, dilution 1:3360) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.

Overview

Product nameDecorin Rabbit mAb
Catalog No.A26852
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC67823
This gene encodes a member of the small leucine-rich proteoglycan family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. This protein plays a role in collagen fibril assembly. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. This gene and the related gene biglycan are thought to be the result of a gene duplication. Mutations in this gene are associated with congenital stromal corneal dystrophy in human patients.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 31-359 of human Decorin (NP_001911.1).
SequenceDEASGIGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQCSDLGLDKVPKDLPPDTTLLDLQNNKITEIKDGDFKNLKNLHALILVNNKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITKVRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMKKLSYIRIADTNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPPGHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRSAIQLGNYK
Gene ID
Swiss Prot
SynonymsCSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
Calculated MW40kDa
Observed MW40-48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:800 - 1:8000
  • IF/ICC 1:3360 - 1:13440
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRat uterus
Cellular locationSecreted, extracellular matrix, extracellular space.

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Certificate of Compliance

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ABclonal:Western blot - Decorin Rabbit mAb (A26852)}

Western blot - Decorin Rabbit mAb (A26852)

Western blot analysis of lysates from Rat uterus using Decorin Rabbit mAb (A26852) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Decorin Rabbit mAb (A26852)}

Immunohistochemistry - Decorin Rabbit mAb (A26852)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Decorin Rabbit mAb (A26852) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Decorin Rabbit mAb (A26852)}

Immunohistochemistry - Decorin Rabbit mAb (A26852)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Decorin Rabbit mAb (A26852) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - Decorin Rabbit mAb (A26852)}

Immunofluorescence - Decorin Rabbit mAb (A26852)

Confocal imaging of paraffin-embedded Human ovary tissue using Decorin Rabbit mAb (A26852, dilution 1:3360) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
ABclonal:Immunofluorescence - Decorin Rabbit mAb (A26852)}

Immunofluorescence - Decorin Rabbit mAb (A26852)

Confocal imaging of paraffin-embedded Human prostate cancer tissue using Decorin Rabbit mAb (A26852, dilution 1:3360) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

ELISA 키트 (1)

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Secondary Antibodies (26)