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DHODH Rabbit pAb (A6899)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DHODH Rabbit pAb (A6899)

Western blot analysis of various lysates using DHODH Rabbit pAb (A6899) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - DHODH Rabbit pAb (A6899)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using DHODH Rabbit pAb (A6899) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - DHODH Rabbit pAb (A6899)

Immunohistochemistry analysis of paraffin-embedded Human esophagus using DHODH Rabbit pAb (A6899) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - DHODH Rabbit pAb (A6899)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using DHODH Rabbit pAb (A6899) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameDHODH Rabbit pAb
Catalog No.A6899
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 116-395 of human DHODH (NP_001352.2).
SequenceEIGSVTPKPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSVVEHRLRARQQKQAKLTEDGLPLGVNLGKNKTSVDAAEDYAEGVRVLGPLADYLVVNVSSPNTAGLRSLQGKAELRRLLTKVLQERDGLRRVHRPAVLVKIAPDLTSQDKEDIASVVKELGIDGLIVTNTTVSRPAGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGRVPIIGVGGVSSGQDALEKIRAGASLVQLYTALTFWGPPVVGKVKRELEALLKEQGFGGVTDAIGADHRR
Gene ID
Swiss Prot
SynonymsURA1; POADS; DHOdehase; DHODH
Calculated MW43kDa
Observed MW43kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMCF7, HL-60, SW620, HepG2, Mouse heart, Mouse spleen, Mouse kidney, Rat heart, Rat kidney
Cellular locationMitochondrion inner membrane, Single-pass membrane protein.
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus)

IF(Mus musculus)

IF(Mus musculus)

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - DHODH Rabbit pAb (A6899)}

    Western blot - DHODH Rabbit pAb (A6899)

    Western blot analysis of various lysates using DHODH Rabbit pAb (A6899) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - DHODH Rabbit pAb (A6899)}

    Immunohistochemistry - DHODH Rabbit pAb (A6899)

    Immunohistochemistry analysis of paraffin-embedded Human liver cancer using DHODH Rabbit pAb (A6899) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - DHODH Rabbit pAb (A6899)}

    Immunohistochemistry - DHODH Rabbit pAb (A6899)

    Immunohistochemistry analysis of paraffin-embedded Human esophagus using DHODH Rabbit pAb (A6899) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - DHODH Rabbit pAb (A6899)}

    Immunohistochemistry - DHODH Rabbit pAb (A6899)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain using DHODH Rabbit pAb (A6899) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A6899? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)