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DHTKD1 Rabbit pAb (A8369)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - DHTKD1 Rabbit pAb (A8369)

Western blot analysis of various lysates, using DHTKD1 Rabbit pAb (A8369) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - DHTKD1 Rabbit pAb (A8369)

Western blot analysis of Rat kidney, using DHTKD1 Rabbit pAb (A8369) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameDHTKD1 Rabbit pAb
Catalog No.A8369
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a component of a mitochondrial 2-oxoglutarate-dehydrogenase-complex-like protein involved in the degradation pathways of several amino acids, including lysine. Mutations in this gene are associated with 2-aminoadipic 2-oxoadipic aciduria and Charcot-Marie-Tooth Disease Type 2Q.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human DHTKD1 (NP_061176.3).
SequenceMASATAAAARRGLGRALPLLWRGYQTERGVYGYRPRKPESREPQGALERPPVDHGLARLVTVYCEHGHKAAKINPLFTGQALLENVPEIQALVQTLQGPFHTAGLLNMGKEEASLEEVLVYLNQIYCGQISIETSQLQSQDEKDWFAKRFEELQKETFTTEERKHLSKLMLESQEFDHFLATKFSTVKRYGGEGAESMMGFFHELLKMSAYSGITDVIIGMPHRGRLNLLTGLLQFPPELMFRKMRGLSEFPENFSATGDVLSHLTSSVDLYFGAHHPLH
Gene ID
Swiss Prot
SynonymsE1a; AAKAD; CMT2Q; AMOXAD; OADC-E1; OADH-E1
Calculated MW103kDa
Observed MW103kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesT-47D, HL-60, Rat kidney
Cellular locationMitochondrion
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - DHTKD1 Rabbit pAb (A8369)}

    Western blot - DHTKD1 Rabbit pAb (A8369)

    Western blot analysis of various lysates, using DHTKD1 Rabbit pAb (A8369) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Western blot - DHTKD1 Rabbit pAb (A8369)}

    Western blot - DHTKD1 Rabbit pAb (A8369)

    Western blot analysis of Rat kidney, using DHTKD1 Rabbit pAb (A8369) at 1:2000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A8369? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)