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DNAJC9 Rabbit pAb (A13176)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - DNAJC9 Rabbit pAb (A13176)

Western blot analysis of various lysates using DNAJC9 Rabbit pAb (A13176) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameDNAJC9 Rabbit pAb
Catalog No.A13176
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables chaperone binding activity; heat shock protein binding activity; and histone binding activity. Involved in nucleosome assembly and positive regulation of ATPase activity. Located in several cellular components, including cytosol; extracellular space; and nucleoplasm. Part of chaperone complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DNAJC9 (NP_056005.1).
SequenceMGLLDLCEEVFGTADLYRVLGVRREASDGEVRRGYHKVSLQVHPDRVGEGDKEDATRRFQILGKVYSVLSDREQRAVYDEQGTVDEDSPVLTQDRDWEAYWRLLFKKISLEDIQAFEKTYKGSEEELADIKQAYLDFKGDMDQIMESVLCVQYTEEPRIRNIIQQAIDAGEVPSYNAFVKESKQKMNARKRRAQEEAKEAEMSRKELGLDEGVDSLKAAIQSRQKDRQKEMDNFLAQMEAKYCKSSKGGGKKSALKKEKK
Gene ID
Swiss Prot
SynonymsJDD1; SB73; HDJC9; DNAJC9
Calculated MW30kDa
Observed MW36kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, OVCAR3, HepG2, HT-29, K-562
Cellular locationCell membrane, Cytoplasm, Nucleus

Documents

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    ABclonal:Western blot - DNAJC9 Rabbit pAb (A13176)}

    Western blot - DNAJC9 Rabbit pAb (A13176)

    Western blot analysis of various lysates using DNAJC9 Rabbit pAb (A13176) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)