제품 > 항체 > 다중클론항체(pAb)

DNMT3A Rabbit pAb (A2065)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using DNMT3A Rabbit pAb (A2065) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunoprecipitation - DNMT3A Rabbit pAb (A2065)

Immunoprecipitation analysis of 200 μg extracts of A-549 cells using 3 μg DNMT3A antibody (A2065). Western blot was performed from the immunoprecipitate using DNMT3A antibody (A2065) at a dilution of 1:1000.

Review

Overview

Product name	DNMT3A Rabbit pAb
Catalog No.	A2065
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-132 of human DNMT3A (NP_072046.2).
Sequence	MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTARKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEGSPAGGQKGGAPAEGEGAAETLPEASRAV
Gene ID
Swiss Prot
Synonyms	TBRS; HESJAS; DNMT3A2; M.HsaIIIA; DNMT3A
Calculated MW	102kDa
Observed MW	110kDa/130kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	A-549, 293T, HeLa, HL-60, MCF7, HepG2, Mouse testis
Cellular location	Cytoplasm, Nucleus
Customer validation	(Human hepatocellular carcinoma cell line, HCT116, Human cells, BEL-7402, SMMC7721) WB(Homo sapiens, Rattus norvegicus, Mus musculus, Other, Oryza sativa) ChIP(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

연구후성유전학 & 핵 신호전달핵심 히스톤의 후성유전적 writer & eraserDNA 메틸화

DNMT3A Rabbit pAb images

Western blot - DNMT3A Rabbit pAb (A2065)

Immunoprecipitation - DNMT3A Rabbit pAb (A2065)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A2065

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Product Protocols

References for DNMT3A Rabbit pAb (A2065)

Product:

DNMT3A Rabbit pAb

Journal:

Neuroscience

Application:

IF:

2.9

Species:

Homo sapiens

PMID:

25779965

Title:

Galangin-induced down-regulation of BACE1 by epigenetic mechanisms in SH-SY5Y cells

References for DNMT3A Rabbit pAb (A2065)

Product:

DNMT3A Rabbit pAb

Journal:

International Journal Of Biochemistry & Cell Biology

Application:

IF:

3.4

Species:

Homo sapiens

PMID:

26299326

Title:

Anticancer drugs induce hypomethylation of the acetylcholinesterase promoter via a phosphorylated-p38-DNMT1-AChE pathway in apoptotic hepatocellular carcinoma cells

References for DNMT3A Rabbit pAb (A2065)

Product:

DNMT3A Rabbit pAb

Journal:

Clinical Epigenetics

Application:

IF:

4.8

Species:

Homo sapiens

PMID:

27034728

Title:

Global histone modification profiling reveals the epigenomic dynamics during malignant transformation in a four-stage breast cancer model

References for DNMT3A Rabbit pAb (A2065)

Product:

DNMT3A Rabbit pAb

Journal:

Front Pharmacol

Application:

IF:

5.6

Species:

Rattus norvegicus

PMID:

33551813

Title:

Role of GABRD Gene Methylation in the Nucleus Accumbens in Heroin-Seeking Behavior in Rats.

References for DNMT3A Rabbit pAb (A2065)

Product:

DNMT3A Rabbit pAb

Journal:

Pharmacological research

Application:

IF:

9.1

Species:

Homo sapiens

PMID:

36464146

Title:

Oroxylin A regulates cGAS DNA hypermethylation induced by methionine metabolism to promote HSC senescence

References for DNMT3A Rabbit pAb (A2065)

Product:

DNMT3A Rabbit pAb

Journal:

Small (Weinheim an der Bergstrasse, Germany)

Application:

IF:

Species:

Mus musculus

PMID:

36403215

Title:

Disturbed Flow‐Facilitated Margination and Targeting of Nanodisks Protect against Atherosclerosis

References for DNMT3A Rabbit pAb (A2065)

Product:

DNMT3A Rabbit pAb

Journal:

Veterinary research

Application:

IF:

3.7

Species:

Homo sapiens

PMID:

37009870

Title:

Host E3 ligase Hrd1 ubiquitinates and degrades H protein of canine distemper virus to inhibit viral replication - PMC

References for DNMT3A Rabbit pAb (A2065)

Product:

DNMT3A Rabbit pAb

Journal:

Biochemical genetics

Application:

IF:

2.1

Species:

Homo sapiens

PMID:

37851210

Title:

M2 Macrophage-Derived Exosomes Regulate miR-199a-3p Promoter Methylation Through the LINC00470-Mediated myc/DNMT3a Axis to Promote Breast Cancer

References for DNMT3A Rabbit pAb (A2065)

Product:

DNMT3A Rabbit pAb

Journal:

International journal of biological macromolecules

Application:

IF:

7.7

Species:

Other

PMID:

38447841

Title:

Genome-wide identification, evolution of DNA methyltransferases and their expression under salinity stress in Larimichthys crocea

References for DNMT3A Rabbit pAb (A2065)

Product:

DNMT3A Rabbit pAb

Journal:

NPJ Regenerative medicine

Application:

IF:

14.4

Species:

Mus musculus

PMID:

37291182

Title:

DNA methyltransferase 1 deficiency improves macrophage motility and wound healing by ameliorating cholesterol accumulation - PMC

References for DNMT3A Rabbit pAb (A2065)

Product:

DNMT3A Rabbit pAb

Journal:

Molecular immunology

Application:

ChIP

IF:

3.2

Species:

Mus musculus

PMID:

37748281

Title:

MiR-4953p attenuates cell pyroptosis and endometritis through inhibiting the activation of NLRP3 inflammasome in bovine

References for DNMT3A Rabbit pAb (A2065)

Product:

DNMT3A Rabbit pAb

Journal:

BMC plant biology

Application:

IF:

4.3

Species:

Oryza sativa

PMID:

39160481

Title:

Spermidine augments salt stress resilience in rice roots potentially by enhancing OsbZIP73s RNA binding capacity - PMC

Publishing research using A2065? Please let us know so that we can cite the reference in this datasheet.

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제품 > 항체 > 다중클론항체(pAb)

DNMT3A Rabbit pAb (A2065)

Overview

Background

Immunogen information

Applications

Documents

Certificate of Compliance

Research Area

DNMT3A Rabbit pAb images

Submit an review for A2065

Reviews List

Product Protocols

항체 (2)

재조합 단백질 (3)

Secondary Antibodies (26)