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DYNLL1 Rabbit pAb (A5742)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - DYNLL1 Rabbit pAb (A5742)

Western blot analysis of extracts of various cell lines, using DYNLL1 antibody (A5742) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Overview

Product nameDYNLL1 Rabbit pAb
Catalog No.A5742
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1, 200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-89 of human DYNLL1 (NP_003737.1).
SequenceMCDRKAVIKNADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG
Gene ID
Swiss Prot
SynonymsLC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1
Calculated MW10kDa
Observed MW13kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMCF7, 293T, HepG2, U-251MG, HeLa, Mouse brain
Cellular locationCytoplasm, Mitochondrion, Nucleus, cytoskeleton
ABclonal:Western blot - DYNLL1 Rabbit pAb (A5742)}

Western blot - DYNLL1 Rabbit pAb (A5742)

Western blot analysis of extracts of various cell lines, using DYNLL1 antibody (A5742) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)