제품 > 항체 > 다중클론항체(pAb)

EBNA1BP2 Rabbit pAb (A9153)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - EBNA1BP2 Rabbit pAb (A9153)

Western blot analysis of extracts of A-431 cells, using EBNA1BP2 antibody (A9153) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - EBNA1BP2 Rabbit pAb (A9153)

Confocal immunofluorescence analysis of U2OS cells using EBNA1BP2 Polyclonal Antibody (A9153) at dilution of 1:200. Blue: DAPI for nuclear staining.

Overview

Product nameEBNA1BP2 Rabbit pAb
Catalog No.A9153
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables RNA binding activity. Predicted to be involved in rRNA processing and ribosomal large subunit biogenesis. Located in chromosome and nucleolus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-306 of human EBNA1BP2 (NP_006815.2).
SequenceMDTPPLSDSESESDESLVTDRELQDAFSRGLLKPGLNVVLEGPKKAVNDVNGLKQCLAEFKRDLEWVERLDVTLGPVPEIGGSEAPAPQNKDQKAVDPEDDFQREMSFYRQAQAAVLAVLPRLHQLKVPTKRPTDYFAEMAKSDLQMQKIRQKLQTKQAAMERSEKAKQLRALRKYGKKVQTEVLQKRQQEKAHMMNAIKKYQKGFSDKLDFLEGDQKPLAQRKKAGAKGQQMRKGPSAKRRYKNQKFGFGGKKKGSKWNTRESYDDVSSFRAKTAHGRGLKRPGKKGSNKRPGKRTREKMKNRTH
Gene ID
Swiss Prot
SynonymsP40; EBP2; NOBP
Calculated MW35kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesA-431
Cellular locationNucleus, nucleolus

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ABclonal:Western blot - EBNA1BP2 Rabbit pAb (A9153)}

Western blot - EBNA1BP2 Rabbit pAb (A9153)

Western blot analysis of extracts of A-431 cells, using EBNA1BP2 antibody (A9153) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - EBNA1BP2 Rabbit pAb (A9153)}

Immunofluorescence - EBNA1BP2 Rabbit pAb (A9153)

Confocal immunofluorescence analysis of U2OS cells using EBNA1BP2 Polyclonal Antibody (A9153) at dilution of 1:200. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)