제품 > 항체 > 다중클론항체(pAb)

ECD Rabbit pAb (A22237)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ECD Rabbit pAb (A22237)

Western blot analysis of HeLa, using ECD antibody (A22237) at 1:800 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - ECD Rabbit pAb (A22237)

Western blot analysis of various lysates, using ECD antibody (A22237) at 1:800 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameECD Rabbit pAb
Catalog No.A22237
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables histone acetyltransferase binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in cytosol and nucleoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 435-644 of human ECD (NP_009196.1).
SequenceESESVSKEEKEQNYDLTEVSESMKAFISKVSTHKGAELPREPSEAPITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDDDLDFETHEPGEEASLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSNILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKPTKN
Gene ID
Swiss Prot
SynonymsGCR2; SGT1; HSGT1
Calculated MW73kDa
Observed MW76kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, Mouse brain, Rat brain
Cellular locationcytoplasm, cytosol, nucleoplasm, nucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - ECD Rabbit pAb (A22237)}

    Western blot - ECD Rabbit pAb (A22237)

    Western blot analysis of HeLa, using ECD antibody (A22237) at 1:800 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - ECD Rabbit pAb (A22237)}

    Western blot - ECD Rabbit pAb (A22237)

    Western blot analysis of various lysates, using ECD antibody (A22237) at 1:800 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)