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EEF1D Rabbit pAb (A2509)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - EEF1D Rabbit pAb (A2509)

Western blot analysis of various lysates using EEF1D Rabbit pAb (A2509) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - EEF1D Rabbit pAb (A2509)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using EEF1D Rabbit pAb (A2509) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - EEF1D Rabbit pAb (A2509)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using EEF1D Rabbit pAb (A2509) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - EEF1D Rabbit pAb (A2509)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using EEF1D Rabbit pAb (A2509) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - EEF1D Rabbit pAb (A2509)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using EEF1D Rabbit pAb (A2509) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - EEF1D Rabbit pAb (A2509)

Immunofluorescence analysis of HeLa cells using EEF1D Rabbit pAb (A2509) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - EEF1D Rabbit pAb (A2509)

Immunofluorescence analysis of PC-12 cells using EEF1D Rabbit pAb (A2509) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - EEF1D Rabbit pAb (A2509)

Immunoprecipitation of EEF1D in 300 µg extracts from jurkat cells using 3 µg EEF1D Rabbit pAb (A2509). Western blot analysis was performed using EEF1D Rabbit pAb (A2509) at 1:500 dilution.

ABclonal:Immunoprecipitation - EEF1D Rabbit pAb (A2509)

Immunoprecipitation of EEF1D in 300 µg extracts from Jurkat cells using 3 µg EEF1D Rabbit pAb (A2509). Western blot analysis was performed using EEF1D Rabbit pAb (A2509) at 1:500 dilution.

Overview

Product nameEEF1D Rabbit pAb
Catalog No.A2509
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human EEF1D (NP_001123529.1).
SequenceMATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLF
Gene ID
Swiss Prot
SynonymsEF1D; EF-1D; FP1047; EEF1D
Calculated MW31kDa
Observed MW40kDa/35kDa/40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesMCF7, Mouse testis, jurkat, Jurkat
Cellular locationNucleus.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - EEF1D Rabbit pAb (A2509)}

    Western blot - EEF1D Rabbit pAb (A2509)

    Western blot analysis of various lysates using EEF1D Rabbit pAb (A2509) at 1:2000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates / proteins: 25 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - EEF1D Rabbit pAb (A2509)}

    Immunohistochemistry - EEF1D Rabbit pAb (A2509)

    Immunohistochemistry analysis of paraffin-embedded Human liver tissue using EEF1D Rabbit pAb (A2509) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - EEF1D Rabbit pAb (A2509)}

    Immunohistochemistry - EEF1D Rabbit pAb (A2509)

    Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using EEF1D Rabbit pAb (A2509) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - EEF1D Rabbit pAb (A2509)}

    Immunohistochemistry - EEF1D Rabbit pAb (A2509)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using EEF1D Rabbit pAb (A2509) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - EEF1D Rabbit pAb (A2509)}

    Immunohistochemistry - EEF1D Rabbit pAb (A2509)

    Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using EEF1D Rabbit pAb (A2509) at a dilution of  1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - EEF1D Rabbit pAb (A2509)}

    Immunofluorescence - EEF1D Rabbit pAb (A2509)

    Immunofluorescence analysis of HeLa cells using EEF1D Rabbit pAb (A2509) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - EEF1D Rabbit pAb (A2509)}

    Immunofluorescence - EEF1D Rabbit pAb (A2509)

    Immunofluorescence analysis of PC-12 cells using EEF1D Rabbit pAb (A2509) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - EEF1D Rabbit pAb (A2509)}

    Immunoprecipitation - EEF1D Rabbit pAb (A2509)

    Immunoprecipitation of EEF1D in 300 µg extracts from jurkat cells using 3 µg EEF1D Rabbit pAb (A2509). Western blot analysis was performed using EEF1D Rabbit pAb (A2509) at 1:500 dilution.
    ABclonal:Immunoprecipitation - EEF1D Rabbit pAb (A2509)}

    Immunoprecipitation - EEF1D Rabbit pAb (A2509)

    Immunoprecipitation of EEF1D in 300 µg extracts from Jurkat cells using 3 µg EEF1D Rabbit pAb (A2509). Western blot analysis was performed using EEF1D Rabbit pAb (A2509) at 1:500 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A2509? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)