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EIF4B Rabbit pAb (A13300)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - EIF4B Rabbit pAb (A13300)

Western blot analysis of lysates from Rat skeletal muscle, using EIF4B Rabbit pAb (A13300) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Western blot - EIF4B Rabbit pAb (A13300)

Western blot analysis of lysates from Mouse kidney, using EIF4B Rabbit pAb (A13300) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - EIF4B Rabbit pAb (A13300)

Immunofluorescence analysis of A-549 cells using EIF4B Rabbit pAb (A13300) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - EIF4B Rabbit pAb (A13300)

Immunofluorescence analysis of NIH/3T3 cells using EIF4B Rabbit pAb (A13300) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - EIF4B Rabbit pAb (A13300)

Immunofluorescence analysis of HeLa cells using EIF4B Rabbit pAb (A13300) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameEIF4B Rabbit pAb
Catalog No.A13300
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables RNA binding activity. Predicted to be involved in eukaryotic translation initiation factor 4F complex assembly and formation of translation preinitiation complex. Located in cytosol. Biomarker of autism spectrum disorder and major depressive disorder.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_001408.2).
SequenceSQSTRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLERRPRERHPSWRSEETQERERSRTGSESSQTGTSTTSSRNARRRESEKSLENETLNKEEDCHSPTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDTEQQSPTSGGGKVAPAQPSEEGPGRKDENKVDGMNAPKGQTGNSSRGPGDGGNRDHWKESDRKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSVDGEDENEGEDYAE
Gene ID
Swiss Prot
SynonymsEIF-4B; PRO1843; EIF4B
Calculated MW69kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse kidney, Rat skeletal muscle
Cellular locationcytosol, eukaryotic translation initiation factor 4F complex.

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ABclonal:Western blot - EIF4B Rabbit pAb (A13300)}

Western blot - EIF4B Rabbit pAb (A13300)

Western blot analysis of lysates from Rat skeletal muscle, using EIF4B Rabbit pAb (A13300) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Western blot - EIF4B Rabbit pAb (A13300)}

Western blot - EIF4B Rabbit pAb (A13300)

Western blot analysis of lysates from Mouse kidney, using EIF4B Rabbit pAb (A13300) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - EIF4B Rabbit pAb (A13300)}

Immunofluorescence - EIF4B Rabbit pAb (A13300)

Immunofluorescence analysis of A-549 cells using EIF4B Rabbit pAb (A13300) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - EIF4B Rabbit pAb (A13300)}

Immunofluorescence - EIF4B Rabbit pAb (A13300)

Immunofluorescence analysis of NIH/3T3 cells using EIF4B Rabbit pAb (A13300) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - EIF4B Rabbit pAb (A13300)}

Immunofluorescence - EIF4B Rabbit pAb (A13300)

Immunofluorescence analysis of HeLa cells using EIF4B Rabbit pAb (A13300) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)