Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human
Western blot analysis of lysates from HeLa cells, using Phospho-EIF4B-S422 Rabbit pAb (AP0775) at 1:2000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
| Product name | Phospho-EIF4B-S422 Rabbit pAb |
|---|---|
| Catalog No. | AP0775 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around S422 of human EIF4B (NP_001408.2). |
|---|---|
| Sequence | TGSES |
| Gene ID | |
| Swiss Prot | |
| Synonyms | EIF-4B; PRO1843; Phospho-EIF4B-S422 |
| Calculated MW | 69kDa |
| Observed MW | 80kDa |
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HeLa treated by EGF |
| Cellular location | cytosol, eukaryotic translation initiation factor 4F complex |
* For research use only. Not for therapeutic or diagnostic purposes.
_WB_01.jpg?t=1717493624 "ABclonal:Western blot - Phospho-EIF4B-S422 Rabbit pAb (AP0775)")
