제품 > 항체 > 다중클론항체(pAb)

EIF4G1 Rabbit pAb (A7552)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from HeLa cells, using EIF4G1 Rabbit pAb (A7552) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - EIF4G1 Rabbit pAb (A7552)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using EIF4G1 Rabbit pAb (A7552) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunoprecipitation - EIF4G1 Rabbit pAb (A7552)

Immunoprecipitation analysis of 300 μg extracts of Hela cells using 3 μg EIF4G1 antibody (A7552). Western blot was performed from the immunoprecipitate using EIF4G1 antibody (A7552) at a dilution of 1:500.

Overview

Product name	EIF4G1 Rabbit pAb
Catalog No.	A7552
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EIF4G1 (NP_886553.3).
Sequence	PEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLE
Gene ID
Swiss Prot
Synonyms	P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1; EIF4G1
Calculated MW	175kDa
Observed MW	220kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500 IHC-P 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	HeLa
Cellular location	cytoplasm, cytoplasmic stress granule, cytosol, eukaryotic translation initiation factor 4F complex, nucleus, postsynapse
Customer validation	WB(Homo sapiens) IF(Homo sapiens)