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Phospho-EIF4G1-S1108 Rabbit pAb (AP0796)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-EIF4G1-S1108 Rabbit pAb (AP0796)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-EIF4G1-S1108 Rabbit pAb (AP0796) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product namePhospho-EIF4G1-S1108 Rabbit pAb
Catalog No.AP0796
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
ImmunogenA synthetic phosphorylated peptide around S1108 of human EIF4G1 (NP_001278086.1).
SequenceSLSRE
Gene ID
Swiss Prot
SynonymsP220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1; Phospho-EIF4G1-S1108
Calculated MW175kDa
Observed MW250kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesNIH/3T3 treated by FBS
Cellular locationcytoplasm, cytoplasmic stress granule, cytosol, eukaryotic translation initiation factor 4F complex, nucleus, postsynapse

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ABclonal:Western blot - Phospho-EIF4G1-S1108 Rabbit pAb (AP0796)}

Western blot - Phospho-EIF4G1-S1108 Rabbit pAb (AP0796)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-EIF4G1-S1108 Rabbit pAb (AP0796) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

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