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ELMO1 Rabbit pAb (A14830)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - ELMO1 Rabbit pAb (A14830)

Western blot analysis of various lysates using ELMO1 Rabbit pAb (A14830) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameELMO1 Rabbit pAb
Catalog No.A14830
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the engulfment and cell motility protein family. These proteins interact with dedicator of cytokinesis proteins to promote phagocytosis and cell migration. Increased expression of this gene and dedicator of cytokinesis 1 may promote glioma cell invasion, and single nucleotide polymorphisms in this gene may be associated with diabetic nephropathy. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 527-727 of human ELMO1 (NP_055615.8).
SequenceDFQSRPILELKEKIQPEILELIKQQRLNRLVEGTCFRKLNARRRQDKFWYCRLSPNHKVLHYGDLEESPQGEVPHDSLQDKLPVADIKAVVTGKDCPHMKEKGALKQNKEVLELAFSILYDSNCQLNFIAPDKHEYCIWTDGLNALLGKDMMSDLTRNDLDTLLSMEIKLRLLDLENIQIPDAPPPIPKEPSNYDFVYDCN
Gene ID
Swiss Prot
SynonymsCED12; CED-12; ELMO-1; ELMO1
Calculated MW84kDa
Observed MW75kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples5637, NCI-H460, BxPC-3, LO2, Mouse eye, Mouse testis
Cellular locationCell membrane, Cytoplasm

Documents

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ABclonal:Western blot - ELMO1 Rabbit pAb (A14830)}

Western blot - ELMO1 Rabbit pAb (A14830)

Western blot analysis of various lysates using ELMO1 Rabbit pAb (A14830) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)