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ENTPD5 Rabbit mAb (A19647)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ENTPD5 Rabbit mAb (A19647)

Western blot analysis of various lysates using ENTPD5 Rabbit mAb (A19647) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - ENTPD5 Rabbit mAb (A19647)

Western blot analysis of various lysates using ENTPD5 Rabbit mAb (A19647) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - ENTPD5 Rabbit mAb (A19647)

Immunohistochemistry analysis of ENTPD5 in paraffin-embedded rat kidney tissue using ENTPD5 Rabbit mAb (A19647) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ENTPD5 Rabbit mAb (A19647)

Immunohistochemistry analysis of ENTPD5 in paraffin-embedded rat liver tissue using ENTPD5 Rabbit mAb (A19647) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ENTPD5 Rabbit mAb (A19647)

Immunohistochemistry analysis of ENTPD5 in paraffin-embedded mouse kidney tissue using ENTPD5 Rabbit mAb (A19647) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ENTPD5 Rabbit mAb (A19647)

Immunohistochemistry analysis of ENTPD5 in paraffin-embedded human small intestine tissue using ENTPD5 Rabbit mAb (A19647) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameENTPD5 Rabbit mAb
Catalog No.A19647
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2211
The protein encoded by this gene is similar to E-type nucleotidases (NTPases)/ecto-ATPase/apyrases. NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD5 contains 4 apyrase-conserved regions which is characteristic of NTPases.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human ENTPD5 (O75356).
SequenceGAETVQGLLEVAKDSIPRSHWKKTPVVLKATAGLRLLPEHKAKALLFEVKEIFRKSPFLVPKGSVSIMDGSDEGILAWVTVNFLTGQLHGHRQETVGTLDL
Gene ID
Swiss Prot
SynonymsPCPH; CD39L4; NTPDase-5; ENTPD5
Calculated MW48kDa
Observed MW48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:3000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, PC-3, Mouse liver, Rat liver, Rat kidney
Cellular locationendoplasmic reticulum, extracellular region, extracellular space

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ABclonal:Western blot - ENTPD5 Rabbit mAb (A19647)}

Western blot - ENTPD5 Rabbit mAb (A19647)

Western blot analysis of various lysates using ENTPD5 Rabbit mAb (A19647) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - ENTPD5 Rabbit mAb (A19647)}

Western blot - ENTPD5 Rabbit mAb (A19647)

Western blot analysis of various lysates using ENTPD5 Rabbit mAb (A19647) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - ENTPD5 Rabbit mAb (A19647)}

Immunohistochemistry - ENTPD5 Rabbit mAb (A19647)

Immunohistochemistry analysis of ENTPD5 in paraffin-embedded rat kidney tissue using ENTPD5 Rabbit mAb (A19647) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ENTPD5 Rabbit mAb (A19647)}

Immunohistochemistry - ENTPD5 Rabbit mAb (A19647)

Immunohistochemistry analysis of ENTPD5 in paraffin-embedded rat liver tissue using ENTPD5 Rabbit mAb (A19647) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ENTPD5 Rabbit mAb (A19647)}

Immunohistochemistry - ENTPD5 Rabbit mAb (A19647)

Immunohistochemistry analysis of ENTPD5 in paraffin-embedded mouse kidney tissue using ENTPD5 Rabbit mAb (A19647) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ENTPD5 Rabbit mAb (A19647)}

Immunohistochemistry - ENTPD5 Rabbit mAb (A19647)

Immunohistochemistry analysis of ENTPD5 in paraffin-embedded human small intestine tissue using ENTPD5 Rabbit mAb (A19647) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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