제품 > 항체 > 다중클론항체(pAb)

EXOSC10 Rabbit pAb (A17519)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - EXOSC10 Rabbit pAb (A17519)

Western blot analysis of various lysates using EXOSC10 Rabbit pAb (A17519) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - EXOSC10 Rabbit pAb (A17519)

Western blot analysis of lysates from Rat brain, using EXOSC10 Rabbit pAb (A17519) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameEXOSC10 Rabbit pAb
Catalog No.A17519
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables 3'-5'-exoribonuclease activity. Involved in several processes, including RNA catabolic process; maturation of 5.8S rRNA; and negative regulation of telomere maintenance via telomerase. Located in cytosol; nuclear lumen; and transcriptionally active chromatin. Part of nuclear exosome (RNase complex).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 586-885 of human EXOSC10 (NP_001001998.1).
SequenceVAAGVKKSGPLPSAERLENVLFGPHDCSHAPPDGYPIIPTSGSVPVQKQASLFPDEKEDNLLGTTCLIATAVITLFNEPSAEDSKKGPLTVAQKKAQNIMESFENPFRMFLPSLGHRAPVSQAAKFDPSTKIYEISNRWKLAQVQVQKDSKEAVKKKAAEQTAAREQAKEACKAAAEQAISVRQQVVLENAAKKRERATSDPRTTEQKQEKKRLKISKKPKDPEPPEKEFTPYDYSQSDFKAFAGNSKSKVSSQFDPNKQTPSGKKCIAAKKIKQSVGNKSMSFPTGKSDRGFRYNWPQR
Gene ID
Swiss Prot
Synonymsp2; p3; p4; RRP6; PMSCL; Rrp6p; PM-Scl; PMSCL2; PM/Scl-100; EXOSC10
Calculated MW101kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-549, Raji, Mouse testis, Mouse spleen, Mouse lung, Rat brain
Cellular locationcytoplasm, cytoplasmic exosome (RNase complex), cytosol, nuclear exosome (RNase complex), nucleolar exosome (RNase complex), nucleolus, nucleoplasm, nucleus

Documents

Certificate of Compliance

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ABclonal:Western blot - EXOSC10 Rabbit pAb (A17519)}

Western blot - EXOSC10 Rabbit pAb (A17519)

Western blot analysis of various lysates using EXOSC10 Rabbit pAb (A17519) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - EXOSC10 Rabbit pAb (A17519)}

Western blot - EXOSC10 Rabbit pAb (A17519)

Western blot analysis of lysates from Rat brain, using EXOSC10 Rabbit pAb (A17519) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)