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EXOSC7 Rabbit mAb (A0501)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - EXOSC7 Rabbit mAb (A0501)

Western blot analysis of extracts of various cell lines, using EXOSC7 antibody (A0501) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameEXOSC7 Rabbit mAb
Catalog No.A0501
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2510
Predicted to enable 3'-5'-exoribonuclease activity and RNA binding activity. Predicted to be involved in RNA metabolic process. Part of exosome (RNase complex).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human EXOSC7 (Q15024).
SequenceMASVTLSEAEKVYIVHGVQEDLRVDGRGCEDYRCVEVETDVVSNTSGSARVKLGHTDILVGVKAEMGTPKLEKPNEGYLEFFVDCSASATPEFEGRGGDD
Gene ID
Swiss Prot
Synonymsp8; EAP1; RRP42; Rrp42p; hRrp42p
Calculated MW32kDa
Observed MW37kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHepG2, A-431, NIH/3T3, K-562
Cellular locationCytoplasm, Nucleus, nucleolus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - EXOSC7 Rabbit mAb (A0501)}

    Western blot - EXOSC7 Rabbit mAb (A0501)

    Western blot analysis of extracts of various cell lines, using EXOSC7 antibody (A0501) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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