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FADD Rabbit PolymAb® (A26418PM)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FADD Rabbit PolymAb® (A26418PM)

Western blot analysis of lysates from Mouse heart using FADD Rabbit PolymAb® (A26418PM) at 1:15000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - FADD Rabbit PolymAb® (A26418PM)

Western blot analysis of various lysates using FADD Rabbit PolymAb® (A26418PM) at 1:15000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - FADD Rabbit PolymAb® (A26418PM)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using FADD Rabbit PolymAb® (A26418PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FADD Rabbit PolymAb® (A26418PM)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using FADD Rabbit PolymAb® (A26418PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FADD Rabbit PolymAb® (A26418PM)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using FADD Rabbit PolymAb® (A26418PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Overview

Product nameFADD Rabbit PolymAb®
Catalog No.A26418PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable several functions, including caspase binding activity; death effector domain binding activity; and tumor necrosis factor receptor superfamily binding activity. Involved in several processes, including hematopoietic or lymphoid organ development; negative regulation of activation-induced cell death of T cells; and positive regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation. Acts upstream of or within extrinsic apoptotic signaling pathway in absence of ligand; motor neuron apoptotic process; and regulation of programmed cell death. Predicted to be located in several cellular components, including cell body; cytosol; and membrane raft. Predicted to be part of CD95 death-inducing signaling complex and ripoptosome. Predicted to be active in cytoplasm. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and liver and biliary system. Human ortholog(s) of this gene implicated in leukemia. Orthologous to human FADD (Fas associated via death domain).
ImmunogenRecombinant fusion protein of human/mouse FADD
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsMort1/FADD
Calculated MW23kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:4500 - 1:15000
  • IHC-P 1:1000 - 1:4000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse heart, HeLa, U-251 MG
Cellular locationcytoplasm, cytosol, neuron projection, nucleus, plasma membrane, ripoptosome.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - FADD Rabbit PolymAb® (A26418PM)}

    Western blot - FADD Rabbit PolymAb® (A26418PM)

    Western blot analysis of lysates from Mouse heart using FADD Rabbit PolymAb® (A26418PM) at 1:15000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - FADD Rabbit PolymAb® (A26418PM)}

    Western blot - FADD Rabbit PolymAb® (A26418PM)

    Western blot analysis of various lysates using FADD Rabbit PolymAb® (A26418PM) at 1:15000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - FADD Rabbit PolymAb® (A26418PM)}

    Immunohistochemistry - FADD Rabbit PolymAb® (A26418PM)

    Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using FADD Rabbit PolymAb® (A26418PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - FADD Rabbit PolymAb® (A26418PM)}

    Immunohistochemistry - FADD Rabbit PolymAb® (A26418PM)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using FADD Rabbit PolymAb® (A26418PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - FADD Rabbit PolymAb® (A26418PM)}

    Immunohistochemistry - FADD Rabbit PolymAb® (A26418PM)

    Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using FADD Rabbit PolymAb® (A26418PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)