제품 > 항체 > 단일클론항체(mAb)

FAK Rabbit mAb (A11131)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using FAK pAb (A11131) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from MCF7 cells, using FAK pAb (A11131) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - FAK Rabbit mAb (A11131)

Confocal imaging of HeLa cells using FAK Rabbit mAb (A11131, at dilution of 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Overview

Product name	FAK Rabbit mAb
Catalog No.	A11131
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0171

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 700-800 of human FAK (Q05397).
Sequence	TVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIG
Gene ID
Swiss Prot
Synonyms	FAK; FADK; FAK1; FRNK; FADK 1; PPP1R71; p125FAK; pp125FAK
Calculated MW	119kDa
Observed MW	125kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	MCF7, Mouse brain, Rat brain
Cellular location	Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Nucleus, Peripheral membrane protein, cell cortex, centrosome, cytoskeleton, focal adhesion, microtubule organizing center
Customer validation	WB(Homo sapiens, Mus musculus) IF(Homo sapiens, Rattus norvegicus) IHC(Homo sapiens) IHC(Rattus norvegicus)

Documents

Certificate of Compliance

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Research Area

FAK Rabbit mAb images

Western blot - FAK Rabbit mAb (A11131)

Immunofluorescence - FAK Rabbit mAb (A11131)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for FAK Rabbit mAb (A11131)

Product:

FAK Rabbit mAb

Journal:

Journal of experimental & clinical cancer research : CR

Application:

IF:

11.161

Species:

Homo sapiens

PMID:

30055645

Title:

Hippo component YAP promotes focal adhesion and tumour aggressiveness via transcriptionally activating THBS1/FAK signalling in breast cancer.

References for FAK Rabbit mAb (A11131)

Product:

FAK Rabbit mAb

Journal:

Cell death & disease

Application:

IF:

Species:

Homo sapiens

PMID:

37460470

Title:

BCKDK regulates breast cancer cell adhesion and tumor metastasis by inhibiting TRIM21 ubiquitinate talin1 - PMC

References for FAK Rabbit mAb (A11131)

Product:

FAK Rabbit mAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Homo sapiens

PMID:

38003505

Title:

Unraveling Connective Tissue Growth Factor as a Therapeutic Target and Assessing Kahweol as a Potential Drug Candidate in Triple-Negative Breast Cancer Treatment - PMC

References for FAK Rabbit mAb (A11131)

Product:

FAK Rabbit mAb

Journal:

PLoS pathogens

Application:

WB, IF

IF:

7.464

Species:

Homo sapiens

PMID:

29415027

Title:

Picornavirus 2A protease regulates stress granule formation to facilitate viral translation

References for FAK Rabbit mAb (A11131)

Product:

FAK Rabbit mAb

Journal:

World journal of gastroenterology

Application:

WB, IHC

IF:

5.37

Species:

Homo sapiens

PMID:

36188720

Title:

Alcohol promotes epithelial mesenchymal transformation-mediated premetastatic niche formation of colorectal cancer by activating interaction between laminin-2 and integrin-1 - PMC

References for FAK Rabbit mAb (A11131)

Product:

FAK Rabbit mAb

Journal:

Stem cell research & therapy

Application:

IF:

8.07

Species:

Homo sapiens

PMID:

35851415

Title:

Extracellular matrix derived from Whartons Jelly-derived mesenchymal stem cells promotes angiogenesis via integrin V3/c-Myc/P300/VEGF - PMC

References for FAK Rabbit mAb (A11131)

Product:

FAK Rabbit mAb

Journal:

Nature communications

Application:

IF:

16.6

Species:

Homo sapiens,Mus musculus

PMID:

38582895

Title:

Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis

References for FAK Rabbit mAb (A11131)

Product:

FAK Rabbit mAb

Journal:

Frontiers in bioengineering and biotechnology

Application:

IHC, IF

IF:

6.06

Species:

Rattus norvegicus

PMID:

36032700

Title:

Effect of viscoelastic properties of cellulose nanocrystal/collagen hydrogels on chondrocyte behaviors - PMC

References for FAK Rabbit mAb (A11131)

Product:

FAK Rabbit mAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.65

Species:

Homo sapiens

PMID:

37269775

Title:

Sophflarine A, a novel matrine-derived alkaloid from Sophora flavescens with therapeutic potential for non-small cell lung cancer through ROS-mediated pyroptosis

References for FAK Rabbit mAb (A11131)

Product:

FAK Rabbit mAb

Journal:

Molecular medicine (Cambridge, Mass.)

Application:

IF:

5.7

Species:

Homo sapiens

PMID:

38698308

Title:

Integrin V3 antagonist-c(RGDyk) peptide attenuates the progression of ossification of the posterior longitudinal ligament by inhibiting osteogenesis and angiogenesis - PMC

References for FAK Rabbit mAb (A11131)

Product:

FAK Rabbit mAb

Journal:

Cell communication and signaling : CCS

Application:

IF:

8.4

Species:

Homo sapiens

PMID:

38816763