제품 > 항체 > 다중클론항체(pAb)

[KD Validated] FAK Rabbit pAb (A11195)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from U-87MG cells, using FAK Rabbit pAb (A11195) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of various lysates using FAK Rabbit pAb (A11195) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from wild type (WT) and FAK knockdown (KD) HeLa cells using FAK Rabbit pAb (A11195) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - [KD Validated] FAK Rabbit pAb (A11195)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using FAK Rabbit pAb (A11195) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using FAK Rabbit pAb (A11195) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using FAK Rabbit pAb (A11195) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using FAK Rabbit pAb (A11195) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain using FAK Rabbit pAb (A11195) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat kidney using FAK Rabbit pAb (A11195) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat lung using FAK Rabbit pAb (A11195) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] FAK Rabbit pAb (A11195)

Immunofluorescence analysis of NIH/3T3 cells using FAK Rabbit pAb (A11195) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using FAK Rabbit pAb (A11195) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using FAK Rabbit pAb (A11195) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using FAK Rabbit pAb (A11195) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using FAK Rabbit pAb (A11195) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using FAK Rabbit pAb (A11195) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	[KD Validated] FAK Rabbit pAb
Catalog No.	A11195
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 734-834 of human FAK (NP_722560.1).
Sequence	QHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLK
Gene ID
Swiss Prot
Synonyms	FAK; FADK; FAK1; FRNK; FADK 1; PPP1R71; p125FAK; pp125FAK
Calculated MW	119kDa
Observed MW	125Kda/119kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	OVCAR3, Mouse testis, Mouse brain, Rat testis, HeLa
Cellular location	Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Nucleus, Peripheral membrane protein, cell cortex, centrosome, cytoskeleton, focal adhesion, microtubule organizing center.
Customer validation	WB(Homo sapiens, Mus musculus) IF(Mus musculus) IHC(Mus musculus, Homo sapiens) IHC(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KD Validated] FAK Rabbit pAb images

Western blot - [KD Validated] FAK Rabbit pAb (A11195)

Immunohistochemistry - [KD Validated] FAK Rabbit pAb (A11195)

Immunofluorescence - [KD Validated] FAK Rabbit pAb (A11195)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KD Validated] FAK Rabbit pAb (A11195)

Product:

[KD Validated] FAK Rabbit pAb

Journal:

Biomaterials

Application:

IF:

12.479

Species:

Homo sapiens

PMID:

32736255

Title:

An exosome-like programmable-bioactivating paclitaxel prodrug nanoplatform for enhanced breast cancer metastasis inhibition

References for [KD Validated] FAK Rabbit pAb (A11195)

Product:

[KD Validated] FAK Rabbit pAb

Journal:

Biomaterials

Application:

IF:

12.479

Species:

Homo sapiens

PMID:

34808560

Title:

ECM stiffness-tuned exosomes drive breast cancer motility through thrombospondin-1

References for [KD Validated] FAK Rabbit pAb (A11195)

Product:

[KD Validated] FAK Rabbit pAb

Journal:

Cancers

Application:

IF:

6.57

Species:

Homo sapiens

PMID:

36010909

Title:

FAM107A Inactivation Associated with Promoter Methylation Affects Prostate Cancer Progression through the FAK/PI3K/AKT Pathway

References for [KD Validated] FAK Rabbit pAb (A11195)

Product:

[KD Validated] FAK Rabbit pAb

Journal:

Bioactive materials

Application:

IF:

16.87

Species:

Mus musculus

PMID:

36817824

Title:

Biomineralization inspired 3D printed bioactive glass nanocomposite scaffolds orchestrate diabetic bone regeneration by remodeling micromilieu - PMC

References for [KD Validated] FAK Rabbit pAb (A11195)

Product:

[KD Validated] FAK Rabbit pAb

Journal:

Cancer science

Application:

IF:

6.51

Species:

Homo sapiens

PMID:

37519194

Title:

Isobutyric acid promotes colorectal cancer metastasis through activating RACK1

References for [KD Validated] FAK Rabbit pAb (A11195)

Product:

[KD Validated] FAK Rabbit pAb

Journal:

Journal of extracellular vesicles

Application:

IF:

17.337

Species:

Homo sapiens, Mus musculus

PMID:

35524442

Title:

Extracellular vesicles rich in HAX1 promote angiogenesis by modulating ITGB6 translation

References for [KD Validated] FAK Rabbit pAb (A11195)

Product:

[KD Validated] FAK Rabbit pAb

Journal:

Cell Adhesion & Migration

Application:

IF:

3.255

Species:

Homo sapiens, Mus musculus

PMID:

31710263

Title:

Paracrine signalling of AGR2 stimulates RhoA function in fibroblasts and modulates cell elongation and migration.

References for [KD Validated] FAK Rabbit pAb (A11195)

Product:

[KD Validated] FAK Rabbit pAb

Journal:

Experimental cell research

Application:

IF:

4.14

Species:

Homo sapiens

PMID:

35513074

Title:

MDM2-mediated ubiquitination of LKB1 contributes to the development of diabetic cataract

References for [KD Validated] FAK Rabbit pAb (A11195)

Product:

[KD Validated] FAK Rabbit pAb

Journal:

Cardiovascular drugs and therapy

Application:

IF:

3.94

Species:

Homo sapiens

PMID:

38363479

Title:

Isoimperatorin Inhibits Angiogenesis by Suppressing VEGFR2 Signaling Pathway

References for [KD Validated] FAK Rabbit pAb (A11195)

Product:

[KD Validated] FAK Rabbit pAb

Journal:

Molecular cancer

Application:

WB, IHC

IF:

41.444

Species:

Mus musculus

PMID:

30458784

Title:

Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK axis.

References for [KD Validated] FAK Rabbit pAb (A11195)

Product:

[KD Validated] FAK Rabbit pAb

Journal:

Cell death discovery

Application:

IHC

IF:

7.109

Species:

Homo sapiens

PMID:

35368030

Title:

CircRPAP2 regulates the alternative splicing of PTK2 by binding to SRSF1 in breast cancer

References for [KD Validated] FAK Rabbit pAb (A11195)

Product:

[KD Validated] FAK Rabbit pAb

Journal:

iScience

Application:

WB, IHC

IF:

6.1

Species:

Homo sapiens

PMID:

37035004

Title:

FSBP suppresses tumor cell migration by inhibiting the JNK pathway - PMC

References for [KD Validated] FAK Rabbit pAb (A11195)

Product:

[KD Validated] FAK Rabbit pAb

Journal:

Heliyon

Application:

IF:

Species:

Homo sapiens

PMID:

39092243

Title:

Enhancing clinical safety in bioengineered-root regeneration: The use of animal component-free medium - PMC

References for [KD Validated] FAK Rabbit pAb (A11195)

Product:

[KD Validated] FAK Rabbit pAb

Journal:

Science advances

Application:

IF:

13.6

Species:

Mus musculus

PMID:

38924414

Title:

Macrophage-derived chemokine CCL22 establishes local LN-mediated adaptive thermogenesis and energy expenditure - PMC

References for [KD Validated] FAK Rabbit pAb (A11195)

Product:

[KD Validated] FAK Rabbit pAb

Journal:

FEBS letters

Application:

IF:

3.5

Species:

Homo sapiens

PMID:

29364502

Title:

ARRDC3 suppresses colorectal cancer progression through destabilizing the oncoprotein YAP

Publishing research using A11195? Please let us know so that we can cite the reference in this datasheet.

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Secondary Antibodies (26)

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제품 > 항체 > 다중클론항체(pAb)

[KD Validated] FAK Rabbit pAb (A11195)

Overview

Background

Immunogen information

Applications

Documents

Certificate of Compliance

Research Area

[KD Validated] FAK Rabbit pAb images

Product Protocols

항체 (6)

Secondary Antibodies (26)