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[KO Validated] Src Rabbit mAb (A19119)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using [KO Validated] Src Rabbit mAb (A19119) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of lysates from wild type (WT) and Src knockout (KO) HeLa cells, using [KO Validated] Src Rabbit mAb (A19119) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

Western blot analysis of lysates from Jurkat cells, using [KO Validated] Src Rabbit mAb (A19119) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

ABclonal:Immunofluorescence - [KO Validated] Src Rabbit mAb (A19119)

Confocal imaging of A549 cells using [KO Validated] Src Rabbit mAb (A19119, at dilution of 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Confocal imaging of NIH/3T3 cells using [KO Validated] Src Rabbit mAb (A19119, at dilution of 1:100) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

ABclonal:Immunoprecipitation - [KO Validated] Src Rabbit mAb (A19119)

Immunoprecipitation analysis of 600 μg extracts of Mouse brain using 3 μg Src antibody (A19119). Western blot was performed from the immunoprecipitate using Src antibody (A19119) at a dilution of 1:1000.

Overview

Product name	[KO Validated] Src Rabbit mAb
Catalog No.	A19119
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0378

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Src (P12931).
Sequence	MGSNKSKPKDASQRRRSLEPAENVHGAGGGAFPASQTPSKPASADGHRGPSAAFAPAAAEPKLFGGFNSSDTVTSPQRAGPLAGGVTTFVALYDYESRTE
Gene ID
Swiss Prot
Synonyms	ASV; SRC1; THC6; c-SRC; p60-Src; rc
Calculated MW	60kDa
Observed MW	60kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IF/ICC 1:100 - 1:400 IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	HeLa, Jurkat, Mouse brain, Mouse lung, Rat brain
Cellular location	Cell membrane, Cytoplasm, Mitochondrion inner membrane, Nucleus, cytoskeleton.
Customer validation	WB(Homo sapiens, Mus musculus, Rattus norvegicus, Other) IF(Homo sapiens) IF(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] Src Rabbit mAb images

Western blot - [KO Validated] Src Rabbit mAb (A19119)

Immunofluorescence - [KO Validated] Src Rabbit mAb (A19119)

Immunoprecipitation - [KO Validated] Src Rabbit mAb (A19119)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] Src Rabbit mAb (A19119)

Product:

[KO Validated] Src Rabbit mAb

Journal:

Cell Biol Toxicol

Application:

IF:

6.691

Species:

Homo sapiens

PMID:

33126266

Title:

Induction of cell cycle arrest and apoptosis by CPUC002 through stabilization of p53 and suppression of STAT3 signaling pathway in multiple myeloma

References for [KO Validated] Src Rabbit mAb (A19119)

Product:

[KO Validated] Src Rabbit mAb

Journal:

Pharmaceuticals (Basel, Switzerland)

Application:

IF:

5.21

Species:

Mus musculus

PMID:

37163367

Title:

Therapeutic Effect of Costunolide in Autoimmune Hepatitis: Network Pharmacology and Experimental Validation - PMC

References for [KO Validated] Src Rabbit mAb (A19119)

Product:

[KO Validated] Src Rabbit mAb

Journal:

Archives of toxicology

Application:

IF:

6.16

Species:

Homo sapiens

PMID:

37407723

Title:

Suppression of sonic hedgehog pathway-based proliferation in glioblastoma cells by small-size silver nanoparticles in vitro

References for [KO Validated] Src Rabbit mAb (A19119)

Product:

[KO Validated] Src Rabbit mAb

Journal:

Cancer cell international

Application:

WB, IF

IF:

6.429

Species:

Homo sapiens

PMID:

35524311

Title:

Tetraspanin 7 promotes osteosarcoma cell invasion and metastasis by inducing EMT and activating the FAK-Src-Ras-ERK1/2 signaling pathway

References for [KO Validated] Src Rabbit mAb (A19119)

Product:

[KO Validated] Src Rabbit mAb

Journal:

Drug development research

Application:

IF:

Species:

Homo sapiens

PMID:

36852868

Title:

Kaempferol suppresses glioma progression and synergistically enhances the antitumor activity of gefitinib by inhibiting the EGFR/SRC/STAT3 signaling pathway

References for [KO Validated] Src Rabbit mAb (A19119)

Product:

[KO Validated] Src Rabbit mAb

Journal:

Cell death & disease

Application:

IF:

Species:

Homo sapiens

PMID:

37612265

Title:

The activated CD36-Src axis promotes lung adenocarcinoma cell proliferation and actin remodeling-involved metastasis in high-fat environment - PMC

References for [KO Validated] Src Rabbit mAb (A19119)

Product:

[KO Validated] Src Rabbit mAb

Journal:

Cardiovascular drugs and therapy

Application:

IF:

3.94

Species:

Homo sapiens

PMID:

38363479

Title:

Isoimperatorin Inhibits Angiogenesis by Suppressing VEGFR2 Signaling Pathway

References for [KO Validated] Src Rabbit mAb (A19119)

Product:

[KO Validated] Src Rabbit mAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

6.54

Species:

Rattus norvegicus

PMID:

35571235

Title:

Characterization of Acetylation of Histone H3 at Lysine 9 in the Trigeminal Ganglion of a Rat Trigeminal Neuralgia Model

References for [KO Validated] Src Rabbit mAb (A19119)

Product:

[KO Validated] Src Rabbit mAb

Journal:

Cell communication and signaling : CCS

Application:

IF:

8.4

Species:

Homo sapiens

PMID:

38816763

Title:

Low concentrations of saracatinib promote definitive endoderm differentiation through inhibition of FAK-YAP signaling axis - PMC

References for [KO Validated] Src Rabbit mAb (A19119)

Product:

[KO Validated] Src Rabbit mAb

Journal:

International journal of molecular sciences

Application:

IF:

5.6

Species:

Other

PMID:

38791533

Title:

Comparison of the Antioxidant and Cytoprotective Properties of Extracts from Different Cultivars of Cornus mas L. - PMC

References for [KO Validated] Src Rabbit mAb (A19119)

Product:

[KO Validated] Src Rabbit mAb

Journal:

Autophagy

Application:

IF:

13.3

Species:

Mus musculus

PMID:

38910554

Title:

(-)-epigallocatechin 3-gallate protects pancreatic -cell against excessive autophagy-induced injury through promoting FTO degradation

References for [KO Validated] Src Rabbit mAb (A19119)

Product:

[KO Validated] Src Rabbit mAb

Journal:

International journal of molecular sciences

Application:

IF:

5.6

Species:

Mus musculus

PMID:

39201475

Title:

Anti-Neuroinflammatory Effect of Ombuin from Rhamnus erythroxylon Pall. Leaves in LPS-Induced BV-2 Microglia by Targeting Src and Suppressing the PI3K-AKT/NF-B Signaling Pathway - PMC

References for [KO Validated] Src Rabbit mAb (A19119)

Product:

[KO Validated] Src Rabbit mAb

Journal:

NPJ Regenerative medicine

Application:

IF:

7.2

Species:

Mus musculus

PMID:

39304660