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Phospho-Src-S17 Rabbit pAb (AP0522)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of extracts of 293T cells, using Phospho-Src-S17 antibody (AP0522) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	Phospho-Src-S17 Rabbit pAb
Catalog No.	AP0522
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S17 of human Phospho-Src-S17 (NP_005408.1).
Sequence	RRSLE
Gene ID
Swiss Prot
Synonyms	ASV; SRC1; THC6; c-SRC; p60-Src
Calculated MW	60kDa
Observed MW	60kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293T
Cellular location	Cell membrane, Cytoplasm, Mitochondrion inner membrane, Nucleus, cytoskeleton
Customer validation	WB(Homo sapiens)