제품 > 항체 > 다중클론항체(pAb)

Src Rabbit pAb (A0324)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from SH-SY5Y cells, using Src Rabbit pAb (A0324) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Review

Overview

Product name	Src Rabbit pAb
Catalog No.	A0324
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 410-536 of human Src (NP_005408.1).
Sequence	LARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL
Gene ID
Swiss Prot
Synonyms	ASV; SRC1; THC6; c-SRC; p60-Src; Src
Calculated MW	60kDa
Observed MW	60kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	SH-SY5Y
Cellular location	Cell membrane, Cytoplasm, Mitochondrion inner membrane, Nucleus, cytoskeleton.
Customer validation	(Human glioma cells) WB(Homo sapiens, Mus musculus, Mus musculus) IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

Src Rabbit pAb images

Western blot - Src Rabbit pAb (A0324)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for Src Rabbit pAb (A0324)

Product:

Src Rabbit pAb

Journal:

Frontiers in Oncology

Application:

IF:

6.244

Species:

Homo sapiens

PMID:

32117782

Title:

PELP1 Suppression Inhibits Gastric Cancer Through Downregulation of c-Src-PI3K-ERK Pathway

References for Src Rabbit pAb (A0324)

Product:

Src Rabbit pAb

Journal:

Pharmacol Res

Application:

IF:

7.658

Species:

Mus musculus

PMID:

32492489

Title:

Oroxylin A suppresses ACTN1 expression to inactivate cancer-associated fibroblasts and restrain breast cancer metastasis

References for Src Rabbit pAb (A0324)

Product:

Src Rabbit pAb

Journal:

Drug Des Devel Ther

Application:

IF:

4.16

Species:

Homo sapiens

PMID:

33833498

Title:

Chlorogenic Acid Inhibits Human Glioma U373 Cell Progression via Regulating the SRC/MAPKs Signal Pathway: Based on Network Pharmacology Analysis

References for Src Rabbit pAb (A0324)

Product:

Src Rabbit pAb

Journal:

Cancer Cell Int

Application:

IF:

5.722

Species:

Homo sapiens

PMID:

34857003

Title:

Transcriptional regulation and ubiquitination-dependent regulation of HnRNPK oncogenic function in prostate tumorigenesis

References for Src Rabbit pAb (A0324)

Product:

Src Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Mus musculus

PMID:

36768559

Title:

Phillygenin Attenuated Colon Inflammation and Improved Intestinal Mucosal Barrier in DSS-induced Colitis Mice via TLR4/Src Mediated MAPK and NF-κB Signaling Pathways - PMC

References for Src Rabbit pAb (A0324)

Product:

Src Rabbit pAb

Journal:

Food Funct

Application:

IF:

6.317

Species:

Homo sapiens

PMID:

33185634

Title:

Polycyclic polyprenylated acylphloroglucinols from Garcinia xanthochymus fruits exhibit antitumor effects by inhibition of STAT3 signaling pathway

References for Src Rabbit pAb (A0324)

Product:

Src Rabbit pAb

Journal:

Journal of translational medicine

Application:

IF:

8.44

Species:

Homo sapiens, Mus musculus

PMID:

36578083

Title:

KPNA2 promotes angiogenesis by regulating STAT3 phosphorylation - PMC

References for Src Rabbit pAb (A0324)

Product:

Src Rabbit pAb

Journal:

Analytical chemistry

Application:

IF:

Species:

Homo sapiens

PMID:

37993803

Title:

Clickable Photoreactive ATP-Affinity Probe for Global Profiling of ATP-Binding Proteins

References for Src Rabbit pAb (A0324)

Product:

Src Rabbit pAb

Journal:

Molecular cancer

Application:

WB, IHC

IF:

41.444

Species:

Mus musculus

PMID:

30458784

Title:

Invasion-related circular RNA circFNDC3B inhibits bladder cancer progression through the miR-1178-3p/G3BP2/SRC/FAK axis.

References for Src Rabbit pAb (A0324)

Product:

Src Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

5.6

Species:

Mus musculus

PMID:

39201475

Title:

Anti-Neuroinflammatory Effect of Ombuin from Rhamnus erythroxylon Pall. Leaves in LPS-Induced BV-2 Microglia by Targeting Src and Suppressing the PI3K-AKT/NF-B Signaling Pathway - PMC